Nosocomial Pseudomonas aeruginosa isolates were typed by DNA fingerprinting techniques. Chromosomal DNA banding patterns, after endonuclease digestion, of six isolates from a neonatal care unit confirmed the results of O-serotyping and antimicrobial susceptibility pattern analysis. In contrast, the characterization by chromosomal DNA fingerprinting of P. aeruginosa strains isolated from patients treated in a burn unit disagreed with the two classic typing methods: in fact, isolates that varied in serotype and antibiograms showed identical restriction endonuclease profiles, whereas two indistinguishable isolates cultured from individual patients were easily and reproducibly differentiated by molecular analysis.
A. NASTASI, C.M. (1989). Molecular study on nosocomial Pseudomonas aeruginosa strains. BOLLETTINO DELL'ISTITUTO SIEROTERAPICO MILANESE, 68(3), 201-205.
Molecular study on nosocomial Pseudomonas aeruginosa strains.
MAMMINA, Caterina;SCARLATA, Giuliana;TORREGROSSA, Maria Valeria;
1989-01-01
Abstract
Nosocomial Pseudomonas aeruginosa isolates were typed by DNA fingerprinting techniques. Chromosomal DNA banding patterns, after endonuclease digestion, of six isolates from a neonatal care unit confirmed the results of O-serotyping and antimicrobial susceptibility pattern analysis. In contrast, the characterization by chromosomal DNA fingerprinting of P. aeruginosa strains isolated from patients treated in a burn unit disagreed with the two classic typing methods: in fact, isolates that varied in serotype and antibiograms showed identical restriction endonuclease profiles, whereas two indistinguishable isolates cultured from individual patients were easily and reproducibly differentiated by molecular analysis.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
