A deep understanding of the molecular interactions of carbon nanodots with biomacromolecules is essential for wider applications of carbon nanodots both in vitro and in vivo. Herein, nitrogen and sulfur co-doped carbon dots (N,S-CDs) with a quantum yield of 16% were synthesized by a 1-step hydrothermal method. The N,S-CDs exhibited a good dispersion, with a graphite-like structure, along with the fluorescence lifetime of approximately 7.50 ns. Findings showed that the fluorescence of the N,S-CDs was effectively quenched by bovine hemoglobin as a result of the static fluorescence quenching. The mentioned quenching mechanism was investigated by the Stern-Volmer equation, temperature-dependent quenching, and fluorescence lifetime measurements. The binding constants, number of binding sites, and the binding average distance between the energy donor N,S-CDs and acceptor bovine hemoglobin were calculated as well. These findings will provide for valuable insights on the future bioapplications of N,S-CDs.

Wang J., Xiang X., Milcovich G., Chen J., Chen C., Feng J., et al. (2019). Nitrogen and sulfur co-doped carbon nanodots toward bovine hemoglobin: A fluorescence quenching mechanism investigation. JOURNAL OF MOLECULAR RECOGNITION, 32(2), 1-9 [10.1002/jmr.2761].

Nitrogen and sulfur co-doped carbon nanodots toward bovine hemoglobin: A fluorescence quenching mechanism investigation

Milcovich G.;
2019-02-01

Abstract

A deep understanding of the molecular interactions of carbon nanodots with biomacromolecules is essential for wider applications of carbon nanodots both in vitro and in vivo. Herein, nitrogen and sulfur co-doped carbon dots (N,S-CDs) with a quantum yield of 16% were synthesized by a 1-step hydrothermal method. The N,S-CDs exhibited a good dispersion, with a graphite-like structure, along with the fluorescence lifetime of approximately 7.50 ns. Findings showed that the fluorescence of the N,S-CDs was effectively quenched by bovine hemoglobin as a result of the static fluorescence quenching. The mentioned quenching mechanism was investigated by the Stern-Volmer equation, temperature-dependent quenching, and fluorescence lifetime measurements. The binding constants, number of binding sites, and the binding average distance between the energy donor N,S-CDs and acceptor bovine hemoglobin were calculated as well. These findings will provide for valuable insights on the future bioapplications of N,S-CDs.
feb-2019
Settore CHIM/09 - Farmaceutico Tecnologico Applicativo
Wang J., Xiang X., Milcovich G., Chen J., Chen C., Feng J., et al. (2019). Nitrogen and sulfur co-doped carbon nanodots toward bovine hemoglobin: A fluorescence quenching mechanism investigation. JOURNAL OF MOLECULAR RECOGNITION, 32(2), 1-9 [10.1002/jmr.2761].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/591734
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