Satellite DNAs such as Cap-A sequences are potentially informative taxonomic and phylogenetic markers useful for characterizing primate genomes. They have also been used as cytogenetic markers facilitating species identification in many taxa. The aim of this work is to map Cap-A sequences by FISH (fluorescent in situ hybridization) on two Platyrrhini (Primates) species genomes, Sapajus cay paraguay and S. macrocephalus, in order to study their distribution pattern on chromosomes. The CapA probes showed bright signals with almost the same interstitial pattern of distribution in correspondence with C and CMA3 rich regions on six pairs of chromosomes in both Sapajus species. An additional pair was detected on S. macrocephalus. The analysis of the results, compared with previous literature data on other phylogenetically close New World species, shows that Cap-A satellite sequences have a genus-specific pattern, but with slight species-specific patterns that are useful as phylogenetic and taxonomic markers.

Simona Ceraulo, Francesca Dumas (2023). Mapping CAP-A satellite DNAs by FISH in Sapajus cay paraguay and S. macrocephalus (Platyrrhini, Primates). CARYOLOGIA, 75(4), 87-92 [10.36253/caryologia-1939].

Mapping CAP-A satellite DNAs by FISH in Sapajus cay paraguay and S. macrocephalus (Platyrrhini, Primates)

Francesca Dumas
2023-04-28

Abstract

Satellite DNAs such as Cap-A sequences are potentially informative taxonomic and phylogenetic markers useful for characterizing primate genomes. They have also been used as cytogenetic markers facilitating species identification in many taxa. The aim of this work is to map Cap-A sequences by FISH (fluorescent in situ hybridization) on two Platyrrhini (Primates) species genomes, Sapajus cay paraguay and S. macrocephalus, in order to study their distribution pattern on chromosomes. The CapA probes showed bright signals with almost the same interstitial pattern of distribution in correspondence with C and CMA3 rich regions on six pairs of chromosomes in both Sapajus species. An additional pair was detected on S. macrocephalus. The analysis of the results, compared with previous literature data on other phylogenetically close New World species, shows that Cap-A satellite sequences have a genus-specific pattern, but with slight species-specific patterns that are useful as phylogenetic and taxonomic markers.
28-apr-2023
Settore BIO/08 - Antropologia
Simona Ceraulo, Francesca Dumas (2023). Mapping CAP-A satellite DNAs by FISH in Sapajus cay paraguay and S. macrocephalus (Platyrrhini, Primates). CARYOLOGIA, 75(4), 87-92 [10.36253/caryologia-1939].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/590939
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