Two distinct types of Polycomb complexes have been identified in flies and in vertebrates, one containing ESC and one containing PC. Using LexA fusions, we show that PC and ESC can establish silencing of a reporter gene but that each requires the presence of the other. In early embryonic extracts, we find PC transiently associated with ESC in a complex that includes EZ, PHO, PH, GAGA, and RPD3 but not PSC. In older embryos, PC is found in a complex including PH, PSC, GAGA, and RPD3, whereas ESC is in a separate complex including EZ, PHO, and RPD3.

Poux S., Melfi R., Pirrotta V. (2001). Establishment of Polycomb silencing requires a transient interaction between PC and ESC. GENES & DEVELOPMENT, 15(19), 2509-2514 [10.1101/gad.208901].

Establishment of Polycomb silencing requires a transient interaction between PC and ESC

Melfi R.
Secondo
Membro del Collaboration Group
;
2001-10-01

Abstract

Two distinct types of Polycomb complexes have been identified in flies and in vertebrates, one containing ESC and one containing PC. Using LexA fusions, we show that PC and ESC can establish silencing of a reporter gene but that each requires the presence of the other. In early embryonic extracts, we find PC transiently associated with ESC in a complex that includes EZ, PHO, PH, GAGA, and RPD3 but not PSC. In older embryos, PC is found in a complex including PH, PSC, GAGA, and RPD3, whereas ESC is in a separate complex including EZ, PHO, and RPD3.
1-ott-2001
Settore BIO/11 - Biologia Molecolare
https://genesdev.cshlp.org/content/15/19/2509
Poux S., Melfi R., Pirrotta V. (2001). Establishment of Polycomb silencing requires a transient interaction between PC and ESC. GENES & DEVELOPMENT, 15(19), 2509-2514 [10.1101/gad.208901].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/587451
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