The production of polyhydroxyalkanoates (PHA) using industrial wastewaters as feedstocks is a current and challenging topic. This study investigated the production of biopolymers by a mixed microbial culture under different OLRs equal to 1 kgCOD m-3d-1 (Period 1), 2 kgCOD m-3d-1 (Period 2) and 3 kgCOD m-3d-1 (Period 3). The maximum PHA content was achieved in Period 2 (0.38 gPHA gTSS-1), whereas lower values were obtained in Period 1 (0.13 gPHA gTSS-1) and Period 3 (0.26 gPHA gTSS-1). Overall, the maximum PHA productivity resulted equal to 0.08 gPHA L-1h-1 (P2), 0.05 gPHA L-1h-1 (P1) and 0.04 gPHA L-1h-1 (P3), respectively. The molecular weight of the PHA increased from Period 1 (250 kDa) to Period 2 (417 KDa) and Period 3 (463 KDa), although resulting in a slight decrease of crystallinity degree. Microbial community analysis, revealed a reduction in bacterial diversity and a progressive shift of the microbial community with the increasing OLR. Alpha-diversity indexes based on Operational Taxonomic Units (OTUs) at 99% identity revealed higher species richness (Taxa (S) 280) and diversity (Shannon (H) 4,06) in Period 1, whereas Period 3 was characterized by reduced richness and diversity and higher dominance (Taxa (S) 133, Shannon (H) 2,40). Based on the results obtained, it was pointed out that the OLR variation determined significant effects on the process performances, as well as on the productivity and quality of the biopolymers. This means that OLR is a key control parameter to maximize the PHA production and control the physical-chemical characteristics of the polymers.

Corsino, S.F., Di Trapani, D., Traina, F., Cruciata, I., Scire Calabrisotto, L., Lopresti, F., et al. (2022). Integrated production of biopolymers with industrial wastewater treatment: Effects of OLR on process yields, biopolymers characteristics and mixed microbial community enrichment. JOURNAL OF WATER PROCESS ENGINEERING, 47 [10.1016/j.jwpe.2022.102772].

Integrated production of biopolymers with industrial wastewater treatment: Effects of OLR on process yields, biopolymers characteristics and mixed microbial community enrichment

Corsino, Santo Fabio
Primo
Methodology
;
Di Trapani, Daniele
Membro del Collaboration Group
;
Traina, Francesco
Formal Analysis
;
Cruciata, Ilenia
Investigation
;
Scire Calabrisotto, Laura
Investigation
;
Lopresti, Francesco
Investigation
;
La Carrubba, Vincenzo
Membro del Collaboration Group
;
Quatrini, Paola
Conceptualization
;
Torregrossa, Michele
Methodology
;
Viviani, Gaspare
Supervision
2022-04-20

Abstract

The production of polyhydroxyalkanoates (PHA) using industrial wastewaters as feedstocks is a current and challenging topic. This study investigated the production of biopolymers by a mixed microbial culture under different OLRs equal to 1 kgCOD m-3d-1 (Period 1), 2 kgCOD m-3d-1 (Period 2) and 3 kgCOD m-3d-1 (Period 3). The maximum PHA content was achieved in Period 2 (0.38 gPHA gTSS-1), whereas lower values were obtained in Period 1 (0.13 gPHA gTSS-1) and Period 3 (0.26 gPHA gTSS-1). Overall, the maximum PHA productivity resulted equal to 0.08 gPHA L-1h-1 (P2), 0.05 gPHA L-1h-1 (P1) and 0.04 gPHA L-1h-1 (P3), respectively. The molecular weight of the PHA increased from Period 1 (250 kDa) to Period 2 (417 KDa) and Period 3 (463 KDa), although resulting in a slight decrease of crystallinity degree. Microbial community analysis, revealed a reduction in bacterial diversity and a progressive shift of the microbial community with the increasing OLR. Alpha-diversity indexes based on Operational Taxonomic Units (OTUs) at 99% identity revealed higher species richness (Taxa (S) 280) and diversity (Shannon (H) 4,06) in Period 1, whereas Period 3 was characterized by reduced richness and diversity and higher dominance (Taxa (S) 133, Shannon (H) 2,40). Based on the results obtained, it was pointed out that the OLR variation determined significant effects on the process performances, as well as on the productivity and quality of the biopolymers. This means that OLR is a key control parameter to maximize the PHA production and control the physical-chemical characteristics of the polymers.
Settore ICAR/03 - Ingegneria Sanitaria-Ambientale
https://www.sciencedirect.com/science/article/pii/S2214714422002161?via=ihub
Corsino, S.F., Di Trapani, D., Traina, F., Cruciata, I., Scire Calabrisotto, L., Lopresti, F., et al. (2022). Integrated production of biopolymers with industrial wastewater treatment: Effects of OLR on process yields, biopolymers characteristics and mixed microbial community enrichment. JOURNAL OF WATER PROCESS ENGINEERING, 47 [10.1016/j.jwpe.2022.102772].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/576649
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