Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).

Francesca Paglia, Elisa Sogne, Davide Panella, Gerardo Perozziello, Carlo Liberale, Gobind Das, et al. (2022). Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells. BIOSENSORS [10.3390/bios12121102].

Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells

Alice Turdo;Simone Di Franco;
2022-12-01

Abstract

Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).
Settore MED/46 - Scienze Tecniche Di Medicina Di Laboratorio
Settore MED/50 - Scienze Tecniche Mediche Applicate
https://www.mdpi.com/2079-6374/12/12/1102
Francesca Paglia, Elisa Sogne, Davide Panella, Gerardo Perozziello, Carlo Liberale, Gobind Das, et al. (2022). Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells. BIOSENSORS [10.3390/bios12121102].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/575108
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