3D printing of polymeric scaffolds and autologous stem cells is a promising tool for damaged facial cartilage reconstruction surgeries. To this end, suitable bioinks are needed to generate scaffolds with the required morphological and functional features. We formulated hydrogel bioinks using k-Carrageen (kC) and poly(vinyl alcohol) (PVA) in three different weight ratios. The kC gives the systems the ability to undergo rapid sol-togel transitions upon cooling from 60°C and above to body temperature, while the PVA is used as rheology modifier and porogen. The latter is crosslinked after molding or printing by freeze-thaw cycling for 1 day (FT1) or 5 days (FT5). To select the most suitable formulation for 3D printing, the sol-to-gel transition and the physico-chemical, mechanical and morphological properties of obtained hydrogels were studied. Moreover, the absence of cytotoxic effects of the material on SASCs was assessed in both stemnesspreserving or chondro-inductive media. Printing trials were performed to identify optimal process parameters and co-printing and post-printing seeding approaches of SASCs were evaluated. Cells were found to be viable after co-printing and also after the FT1 treatment. Viable adherent cells were also found in the FT5 system, where cells were plated after freezing and thawing treatment.

Emanuela Muscolino, A.B.D.S. (2022). κ-Carrageenan and PVA blends as bioinks to 3D print scaffolds for cartilage reconstruction. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 222, 1861-1875 [10.1016/j.ijbiomac.2022.09.275].

κ-Carrageenan and PVA blends as bioinks to 3D print scaffolds for cartilage reconstruction

Emanuela Muscolino;Anna Barbara Di Stefano
Co-primo
;
Marco Trapani;Maria Antonietta Sabatino;Sabina Alessi;Emanuele Cammarata;Francesco Moschella;Adriana Cordova;Francesca Toia;Clelia Dispenza
2022-12-01

Abstract

3D printing of polymeric scaffolds and autologous stem cells is a promising tool for damaged facial cartilage reconstruction surgeries. To this end, suitable bioinks are needed to generate scaffolds with the required morphological and functional features. We formulated hydrogel bioinks using k-Carrageen (kC) and poly(vinyl alcohol) (PVA) in three different weight ratios. The kC gives the systems the ability to undergo rapid sol-togel transitions upon cooling from 60°C and above to body temperature, while the PVA is used as rheology modifier and porogen. The latter is crosslinked after molding or printing by freeze-thaw cycling for 1 day (FT1) or 5 days (FT5). To select the most suitable formulation for 3D printing, the sol-to-gel transition and the physico-chemical, mechanical and morphological properties of obtained hydrogels were studied. Moreover, the absence of cytotoxic effects of the material on SASCs was assessed in both stemnesspreserving or chondro-inductive media. Printing trials were performed to identify optimal process parameters and co-printing and post-printing seeding approaches of SASCs were evaluated. Cells were found to be viable after co-printing and also after the FT1 treatment. Viable adherent cells were also found in the FT5 system, where cells were plated after freezing and thawing treatment.
https://www.sciencedirect.com/science/article/pii/S0141813022022152
Emanuela Muscolino, A.B.D.S. (2022). κ-Carrageenan and PVA blends as bioinks to 3D print scaffolds for cartilage reconstruction. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 222, 1861-1875 [10.1016/j.ijbiomac.2022.09.275].
File in questo prodotto:
File Dimensione Formato  
23- IJBIOMAC 2022_compressed.pdf

Solo gestori archvio

Tipologia: Post-print
Dimensione 1.94 MB
Formato Adobe PDF
1.94 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
1-s2.0-S0141813022022152_compressed.pdf

Solo gestori archvio

Tipologia: Versione Editoriale
Dimensione 989.84 kB
Formato Adobe PDF
989.84 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/572487
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 1
social impact