Olive (Olea europaea L.) is an ancient crop which spread from the Middle East towards the westem Mediterranean region. In addition lo its agronomical and economical importance, the olive industry has a great social and cultural interest as part of our heritage. In order to characterize and to study the genetic relationship among cultivars, five polymorphic microsatellite loci were used in this study and were generated a total of 56 polymorphic alleles with an average of 11 alleles by locus. For all genotypes, the highest number of polymorphic alleles was obtained by the locus UD0043 and GAPU 103. The polymorphism index content ranged from 0.836 for the locus ssrOeUADCA-04 and 0.919 for the locus GAPU-103. The analysis of the dendrogram and the principal coordinates analysis produced by SSR markers grouped studied cultivars into four distinct clusters by cutting the dendrogram at genetic similarity value of 0. 1. Accessions of 'Marsaline', Fouji' and 'Fokhari' are grouped in one clear cluster. 'Zarrazi' and 'Meski' varieties are grouped in separated clear class. The last group was formed by 'Picholine' and 'Chemcheli' varieties.
Abdelhamid, S., Grati-Kamoun, N., Marra, F.P., Caruso, T. (2010). Genetic Characterization of Tunisian Olive Table Cultivars (Olea europaea L.): Inventory Based on Microsatellite Analysis. THE AFRICAN JOURNAL OF PLANT SCIENCE AND BIOTECHNOLOGY, 4 (Special Issue 2)(4), 13-16.
Genetic Characterization of Tunisian Olive Table Cultivars (Olea europaea L.): Inventory Based on Microsatellite Analysis
MARRA, Francesco Paolo;CARUSO, Tiziano
2010-01-01
Abstract
Olive (Olea europaea L.) is an ancient crop which spread from the Middle East towards the westem Mediterranean region. In addition lo its agronomical and economical importance, the olive industry has a great social and cultural interest as part of our heritage. In order to characterize and to study the genetic relationship among cultivars, five polymorphic microsatellite loci were used in this study and were generated a total of 56 polymorphic alleles with an average of 11 alleles by locus. For all genotypes, the highest number of polymorphic alleles was obtained by the locus UD0043 and GAPU 103. The polymorphism index content ranged from 0.836 for the locus ssrOeUADCA-04 and 0.919 for the locus GAPU-103. The analysis of the dendrogram and the principal coordinates analysis produced by SSR markers grouped studied cultivars into four distinct clusters by cutting the dendrogram at genetic similarity value of 0. 1. Accessions of 'Marsaline', Fouji' and 'Fokhari' are grouped in one clear cluster. 'Zarrazi' and 'Meski' varieties are grouped in separated clear class. The last group was formed by 'Picholine' and 'Chemcheli' varieties.
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