Guanosine (GUO), widely considered a key signaling mediator, is implicated in the regulation of several cellular processes. While its interaction with neural membranes has been described, GUO still is an orphan neuromodulator. It has been postulated that GUO may eventually interact with potassium channels and adenosine (ADO) receptors (ARs), both particularly important for the control of cellular excitability. Accordingly, here, we investigated the efects of GUO on the bioelectric activity of human neuroblastoma SH-SY5Y cells by whole-cell patch-clamp recordings. We frst explored the contribution of voltage-dependent K+ channels and, besides this, the role of ARs in the regulation of GUO-dependent cellular electrophysiology. Our data support that GUO is able to specifcally modulate K+-dependent outward currents over cell membranes. Importantly, administering ADO along with GUO potentiates its efects. Overall, these results suggested that K+ outward membrane channels may be targeted by GUO with an implication of ADO receptors in SH-SY5Y cells, but also support the hypothesis of a functional interaction of the two ligands. The present research runs through the leitmotif of the deorphanization of GUO, adding insight on the interplay with adenosinergic signaling and suggesting GUO as a powerful modulator of SH-SY5Y excitability.

Gambino, G., Giglia, G., Gallo, D., Scordino, M., Giardina, C., Zuccarini, M., et al. (2022). Guanosine modulates K+ membrane currents in SH-SY5Y cells: involvement of adenosine receptors. PFLÜGERS ARCHIV [10.1007/s00424-022-02741-4].

Guanosine modulates K+ membrane currents in SH-SY5Y cells: involvement of adenosine receptors

Gambino, Giuditta
;
Giglia, Giuseppe
Co-primo
;
Gallo, Daniele;Scordino, Miriana;Giardina, Costanza;Ferraro, Giuseppe;Mudo', Giuseppa;Sardo, Pierangelo;Di Liberto, Valentina
2022-09-01

Abstract

Guanosine (GUO), widely considered a key signaling mediator, is implicated in the regulation of several cellular processes. While its interaction with neural membranes has been described, GUO still is an orphan neuromodulator. It has been postulated that GUO may eventually interact with potassium channels and adenosine (ADO) receptors (ARs), both particularly important for the control of cellular excitability. Accordingly, here, we investigated the efects of GUO on the bioelectric activity of human neuroblastoma SH-SY5Y cells by whole-cell patch-clamp recordings. We frst explored the contribution of voltage-dependent K+ channels and, besides this, the role of ARs in the regulation of GUO-dependent cellular electrophysiology. Our data support that GUO is able to specifcally modulate K+-dependent outward currents over cell membranes. Importantly, administering ADO along with GUO potentiates its efects. Overall, these results suggested that K+ outward membrane channels may be targeted by GUO with an implication of ADO receptors in SH-SY5Y cells, but also support the hypothesis of a functional interaction of the two ligands. The present research runs through the leitmotif of the deorphanization of GUO, adding insight on the interplay with adenosinergic signaling and suggesting GUO as a powerful modulator of SH-SY5Y excitability.
set-2022
Settore BIO/09 - Fisiologia
Gambino, G., Giglia, G., Gallo, D., Scordino, M., Giardina, C., Zuccarini, M., et al. (2022). Guanosine modulates K+ membrane currents in SH-SY5Y cells: involvement of adenosine receptors. PFLÜGERS ARCHIV [10.1007/s00424-022-02741-4].
File in questo prodotto:
File Dimensione Formato  
Gambino2022 Euro J Physio.pdf

accesso aperto

Tipologia: Versione Editoriale
Dimensione 3.47 MB
Formato Adobe PDF
3.47 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/567662
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact