Aim The effects of local applied NO-active compounds on glutamate (GLU)-evoked responses were investigated in globus pallidus (GP) neurons. Main methods Extracellularly recorded single units from anesthetized rats were treated with GLU before and during the microiontophoretic application of S-nitrosoglutathione (SNOG), a NO donor, and Nω-nitro-l-arginine methyl ester (L-NAME), a NOS inhibitor. Key findings Most GP cells were excited by SNOG whereas administration of L-NAME induced decrease of GP neurons activity. Nearly all neurons responding to SNOG and/or L-NAME showed significant modulation of their excitatory responses to the administration of iontophoretic GLU. In these cells, the changes induced by NO-active drugs in the magnitude of GLU-evoked responses were used as indicators of NO modulation. In fact, when a NO-active drug was co-iontophoresed with GLU, significant changes in GLU-induced responses were observed: generally, increased magnitudes of GLU-evoked responses were observed during SNOG ejection, whereas the administration of L-NAME decreased responses to GLU. Significance The results suggest that the NO-active drugs modulate the response of GP neurons to glutamatergic transmission. Nitrergic modulation of glutamatergic transmission could play an important role in the control of GP bioelectric activity, considered a fundamental key in the BG function.

Sardo, P., Carletti, F., Rizzo, V., Lonobile, G., Friscia, S., Ferraro, G. (2011). Nitric oxide-active compounds modulate the intensity of glutamate-evoked responses in the globus pallidus of the rat. LIFE SCIENCES, 88, 1113-1120 [10.1016/j.lfs.2011.04.010].

Nitric oxide-active compounds modulate the intensity of glutamate-evoked responses in the globus pallidus of the rat

SARDO, Pierangelo;CARLETTI, Fabio;RIZZO, Valerio;FRISCIA, Simonetta;FERRARO, Giuseppe
2011-01-01

Abstract

Aim The effects of local applied NO-active compounds on glutamate (GLU)-evoked responses were investigated in globus pallidus (GP) neurons. Main methods Extracellularly recorded single units from anesthetized rats were treated with GLU before and during the microiontophoretic application of S-nitrosoglutathione (SNOG), a NO donor, and Nω-nitro-l-arginine methyl ester (L-NAME), a NOS inhibitor. Key findings Most GP cells were excited by SNOG whereas administration of L-NAME induced decrease of GP neurons activity. Nearly all neurons responding to SNOG and/or L-NAME showed significant modulation of their excitatory responses to the administration of iontophoretic GLU. In these cells, the changes induced by NO-active drugs in the magnitude of GLU-evoked responses were used as indicators of NO modulation. In fact, when a NO-active drug was co-iontophoresed with GLU, significant changes in GLU-induced responses were observed: generally, increased magnitudes of GLU-evoked responses were observed during SNOG ejection, whereas the administration of L-NAME decreased responses to GLU. Significance The results suggest that the NO-active drugs modulate the response of GP neurons to glutamatergic transmission. Nitrergic modulation of glutamatergic transmission could play an important role in the control of GP bioelectric activity, considered a fundamental key in the BG function.
Settore BIO/09 - Fisiologia
Sardo, P., Carletti, F., Rizzo, V., Lonobile, G., Friscia, S., Ferraro, G. (2011). Nitric oxide-active compounds modulate the intensity of glutamate-evoked responses in the globus pallidus of the rat. LIFE SCIENCES, 88, 1113-1120 [10.1016/j.lfs.2011.04.010].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/54399
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