Human telomeric DNA, in G-quadruplex (G4) conformation, is characterized by a remarkable structural stability that confers it the capacity to resist to oxidative stress producing one or even clustered 8-oxoguanine (8oxoG) lesions. We present a combined experimental/computational investigation, by using circular dichroism in aqueous solutions, cellular immunofluorescence assays and molecular dynamics simulations, that identifies the crucial role of the stability of G4s to oxidative lesions, related also to their biological role as inhibitors of telomerase, an enzyme overexpressed in most cancers associated to oxidative stress.

Miclot T., Corbier C., Terenzi A., Hognon C., Grandemange S., Barone G., et al. (2021). Forever Young: Structural Stability of Telomeric Guanine Quadruplexes in the Presence of Oxidative DNA Lesions**. CHEMISTRY-A EUROPEAN JOURNAL, 27(34), 8865-8874 [10.1002/chem.202100993].

Forever Young: Structural Stability of Telomeric Guanine Quadruplexes in the Presence of Oxidative DNA Lesions**

Miclot T.
Membro del Collaboration Group
;
Terenzi A.
Membro del Collaboration Group
;
Barone G.
Membro del Collaboration Group
;
2021-06-16

Abstract

Human telomeric DNA, in G-quadruplex (G4) conformation, is characterized by a remarkable structural stability that confers it the capacity to resist to oxidative stress producing one or even clustered 8-oxoguanine (8oxoG) lesions. We present a combined experimental/computational investigation, by using circular dichroism in aqueous solutions, cellular immunofluorescence assays and molecular dynamics simulations, that identifies the crucial role of the stability of G4s to oxidative lesions, related also to their biological role as inhibitors of telomerase, an enzyme overexpressed in most cancers associated to oxidative stress.
16-giu-2021
Settore CHIM/03 - Chimica Generale E Inorganica
Miclot T., Corbier C., Terenzi A., Hognon C., Grandemange S., Barone G., et al. (2021). Forever Young: Structural Stability of Telomeric Guanine Quadruplexes in the Presence of Oxidative DNA Lesions**. CHEMISTRY-A EUROPEAN JOURNAL, 27(34), 8865-8874 [10.1002/chem.202100993].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/513790
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