In this work, we introduce the use of 4-dimethylamino-4′-nitrostilbene (DANS) fluorescent dye for applications in the detection and analysis of microplastics, an impendent source of pollution made of synthetic organic polymers with a size varying from less than 5 mm to nanometer scale. The use of this dye revealed itself as a versatile, fast and sensitive tool for readily discriminate microplastics in water environment. The experimental evidences herein presented demonstrate that DANS efficiently absorbs into a variety of polymers constituting microplastics, and its solvatochromic properties lead to a positive shift of the fluorescence emission spectrum according to the polarity of the polymers. Therefore, under UV illumination, microplastics glow a specific emission spectrum from blue to red that allows for a straightforward polymer identification. In addition, we show that DANS staining gives access to different detection and analysis strategies based on fluorescence microscopy, from simple epifluorescence fragments visualization, to confocal microscopy and phasor approach for plastic components quantification.

Sancataldo G., Ferrara V., Bonomo F.P., Chillura Martino D.F., Licciardi M., Pignataro B.G., et al. (2021). Identification of microplastics using 4-dimethylamino-4′-nitrostilbene solvatochromic fluorescence. MICROSCOPY RESEARCH AND TECHNIQUE, 1-12 [10.1002/jemt.23841].

Identification of microplastics using 4-dimethylamino-4′-nitrostilbene solvatochromic fluorescence

Sancataldo G.
;
Ferrara V.;Bonomo F. P.;Chillura Martino D. F.;Licciardi M.;Pignataro B. G.;Vetri V.
2021

Abstract

In this work, we introduce the use of 4-dimethylamino-4′-nitrostilbene (DANS) fluorescent dye for applications in the detection and analysis of microplastics, an impendent source of pollution made of synthetic organic polymers with a size varying from less than 5 mm to nanometer scale. The use of this dye revealed itself as a versatile, fast and sensitive tool for readily discriminate microplastics in water environment. The experimental evidences herein presented demonstrate that DANS efficiently absorbs into a variety of polymers constituting microplastics, and its solvatochromic properties lead to a positive shift of the fluorescence emission spectrum according to the polarity of the polymers. Therefore, under UV illumination, microplastics glow a specific emission spectrum from blue to red that allows for a straightforward polymer identification. In addition, we show that DANS staining gives access to different detection and analysis strategies based on fluorescence microscopy, from simple epifluorescence fragments visualization, to confocal microscopy and phasor approach for plastic components quantification.
Settore CHIM/02 - Chimica Fisica
Settore CHIM/09 - Farmaceutico Tecnologico Applicativo
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Sancataldo G., Ferrara V., Bonomo F.P., Chillura Martino D.F., Licciardi M., Pignataro B.G., et al. (2021). Identification of microplastics using 4-dimethylamino-4′-nitrostilbene solvatochromic fluorescence. MICROSCOPY RESEARCH AND TECHNIQUE, 1-12 [10.1002/jemt.23841].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/512783
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