B lymphocytes are among the cell types whose effector functions are modulated by mast cells (MCs). The B/MC crosstalk emerged in several pathological settings, notably the colon of inflammatory bowel disease (IBD) patients is a privileged site in which MCs and IgA+ cells physically interact. Herein, by inducing conditional depletion of MCs in red MC and basophil (RMB) mice, we show that MCs control B cell distribution in the gut and IgA serum levels. Moreover, in dextran sulfate sodium (DSS)-treated RMB mice, the presence of MCs is fundamental for the enlargement of the IgA+ population in the bowel and the increase of systemic IgA production. Since both conventional B-2 and peritoneal-derived B cells populate the intestine and communicate with MCs in physiological conditions and during inflammation, we further explored this interplay through the use of co-cultures. We show that MCs finely regulate different aspects of splenic B cell biology while peritoneal B cells are unresponsive to the supporting effects provided by MCs. Interestingly, peritoneal B cells induce a pro-inflammatory skewing in MCs, characterized by increased ST2 and TNF-α expression. Altogether, this study uncovers the versatility of the B/MC liaison and highlights key aspects for the resolution of intestinal inflammation.

Valeri V., Tonon S., Vibhushan S., Gulino A., Belmonte B., Adori M., et al. (2021). Mast cells crosstalk with B cells in the gut and sustain IgA response in the inflamed intestine. EUROPEAN JOURNAL OF IMMUNOLOGY, 51(2), 445-458 [10.1002/eji.202048668].

Mast cells crosstalk with B cells in the gut and sustain IgA response in the inflamed intestine

Gulino A.;Belmonte B.;Tripodo C.;
2021-01-01

Abstract

B lymphocytes are among the cell types whose effector functions are modulated by mast cells (MCs). The B/MC crosstalk emerged in several pathological settings, notably the colon of inflammatory bowel disease (IBD) patients is a privileged site in which MCs and IgA+ cells physically interact. Herein, by inducing conditional depletion of MCs in red MC and basophil (RMB) mice, we show that MCs control B cell distribution in the gut and IgA serum levels. Moreover, in dextran sulfate sodium (DSS)-treated RMB mice, the presence of MCs is fundamental for the enlargement of the IgA+ population in the bowel and the increase of systemic IgA production. Since both conventional B-2 and peritoneal-derived B cells populate the intestine and communicate with MCs in physiological conditions and during inflammation, we further explored this interplay through the use of co-cultures. We show that MCs finely regulate different aspects of splenic B cell biology while peritoneal B cells are unresponsive to the supporting effects provided by MCs. Interestingly, peritoneal B cells induce a pro-inflammatory skewing in MCs, characterized by increased ST2 and TNF-α expression. Altogether, this study uncovers the versatility of the B/MC liaison and highlights key aspects for the resolution of intestinal inflammation.
Settore MED/08 - Anatomia Patologica
Valeri V., Tonon S., Vibhushan S., Gulino A., Belmonte B., Adori M., et al. (2021). Mast cells crosstalk with B cells in the gut and sustain IgA response in the inflamed intestine. EUROPEAN JOURNAL OF IMMUNOLOGY, 51(2), 445-458 [10.1002/eji.202048668].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/510018
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