The involvement of non-canonical DNA structures, such as Gquadruplex (G4) DNA, in cancer development and progression has set the pace towards the renaissance of DNA-binding metal complexes. In this work, we report the DNA-binding of three Ni(II), Cu(II), Zn(II) complexes of a salphen-like N4-donor ligand, bearing two imidazole groups condensed with a phenylenediamine moiety. Both duplex and G4 DNAs derived from human telomeres (hTelo), and a sequence mimicking the promoter of the oncogene myc (c-myc) were studied. UV-Vis and circular dichroism spectroscopic binding studies pointed out that, while all the three complexes bind the selected oligonucleotides, the Cu(II) derivative is the strongest and G4-selective compound of the series. Lastly, FRET DNA melting assay results on the Cu(II) complex/hTelo G4 system were interpreted by a loop-binding mechanism of interaction, as corroborated by molecular dynamics (MD) simulations.

Farine, G., Migliore, C., Terenzi, A., Lo Celso, F., Santoro, A., Bruno, G., et al. (2021). On the G‐quadruplex binding of a new class of Ni(II), Cu(II) and Zn(II) salphen‐like complexes. EUROPEAN JOURNAL OF INORGANIC CHEMISTRY(14), 1-6 [10.1002/ejic.202100067].

On the G‐quadruplex binding of a new class of Ni(II), Cu(II) and Zn(II) salphen‐like complexes

Farine, Gianluca
Membro del Collaboration Group
;
Terenzi, Alessio
Membro del Collaboration Group
;
Lo Celso, Fabrizio
Membro del Collaboration Group
;
Bonsignore, Riccardo
Membro del Collaboration Group
;
Barone, Giampaolo
Membro del Collaboration Group
2021

Abstract

The involvement of non-canonical DNA structures, such as Gquadruplex (G4) DNA, in cancer development and progression has set the pace towards the renaissance of DNA-binding metal complexes. In this work, we report the DNA-binding of three Ni(II), Cu(II), Zn(II) complexes of a salphen-like N4-donor ligand, bearing two imidazole groups condensed with a phenylenediamine moiety. Both duplex and G4 DNAs derived from human telomeres (hTelo), and a sequence mimicking the promoter of the oncogene myc (c-myc) were studied. UV-Vis and circular dichroism spectroscopic binding studies pointed out that, while all the three complexes bind the selected oligonucleotides, the Cu(II) derivative is the strongest and G4-selective compound of the series. Lastly, FRET DNA melting assay results on the Cu(II) complex/hTelo G4 system were interpreted by a loop-binding mechanism of interaction, as corroborated by molecular dynamics (MD) simulations.
Settore CHIM/03 - Chimica Generale E Inorganica
Farine, G., Migliore, C., Terenzi, A., Lo Celso, F., Santoro, A., Bruno, G., et al. (2021). On the G‐quadruplex binding of a new class of Ni(II), Cu(II) and Zn(II) salphen‐like complexes. EUROPEAN JOURNAL OF INORGANIC CHEMISTRY(14), 1-6 [10.1002/ejic.202100067].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/492617
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