COX- Expression in the inflamed gingival tissue:Immunohistochemistry,Cytochemistry and Biochemistry test.

Prostaglandins (PG) are chemical mediators for most physiological and pathological responses ,including cytoprotection of the gastric mucosa, blood coagulation,inflammation and pain (1,2,3). E2 Prostaglandins (PG2) play an important role of pathogenesis of periodontal diseases, since PG2 determines vasodilatation and increased vascular permeability, oedema and erythema.Therefore,it is not surprising to find high levels of PG2 in inflamed gingival tissues.Indeed ,in inflamed gingival tissue,PG2 levels of the Gingival Fluid (GCF) are known to be higher in periodontitis than in healthy gingiva.Moreover ,in different parodontitis the high PG2 level of the GCF is related to periodontal ligament loss.The pathogenesis of the periodontitis can be due to the high PG2activation of the Matrix Metal-Protease (MMP),which is synthesized by monocites and fibroblasts.Consequently,destruction of connective tissue on a large scale takes place.The aim of this research is two-fold: To test COX-2 expression in the human espulsi and study its involvement in the PG2 synthesis and to find a alternative therapy to treat gingival diseases.