Confocal detection in digital scanned laser light-sheet fluorescence microscopy (DSLM) has been established as a gold standard method to improve image quality. The selective line detection of a complementary metal-oxide-semiconductor camera (CMOS) working in rolling shutter mode allows the rejection of out-of-focus and scattered light, thus reducing background signal during image formation. Most modern CMOS have two rolling shutters, but usually only a single illuminating beam is used, halving the maximum obtainable frame rate. We report on the capability to recover the full image acquisition rate via dual confocal DSLM by using an acoustooptic deflector. Such a simple solution enables us to independently generate, control and synchronize two beams with the two rolling slits on the camera. We show that the doubling of the imaging speed does not affect the confocal detection high contrast. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.

Gavryusev V, Sancataldo G, Ricci P, Montalbano A, Fornetto C, Turrini L, et al. (2019). Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector. JOURNAL OF BIOMEDICAL OPTICS, 24(10), 106504 [10.1117/1.JBO.24.10.106504].

Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector

Sancataldo G;
2019

Abstract

Confocal detection in digital scanned laser light-sheet fluorescence microscopy (DSLM) has been established as a gold standard method to improve image quality. The selective line detection of a complementary metal-oxide-semiconductor camera (CMOS) working in rolling shutter mode allows the rejection of out-of-focus and scattered light, thus reducing background signal during image formation. Most modern CMOS have two rolling shutters, but usually only a single illuminating beam is used, halving the maximum obtainable frame rate. We report on the capability to recover the full image acquisition rate via dual confocal DSLM by using an acoustooptic deflector. Such a simple solution enables us to independently generate, control and synchronize two beams with the two rolling slits on the camera. We show that the doubling of the imaging speed does not affect the confocal detection high contrast. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
Gavryusev V, Sancataldo G, Ricci P, Montalbano A, Fornetto C, Turrini L, et al. (2019). Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector. JOURNAL OF BIOMEDICAL OPTICS, 24(10), 106504 [10.1117/1.JBO.24.10.106504].
File in questo prodotto:
File Dimensione Formato  
106504_1.pdf

accesso aperto

Tipologia: Versione Editoriale
Dimensione 2.74 MB
Formato Adobe PDF
2.74 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/468643
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 14
social impact