Brain function emerges from the coordinated activity, over time, of large neuronal populations placed in different brain regions. Understanding the relationships of these specific areas and disentangling the contributions of individual neurons to overall function remain central goals for neuroscience. In this scenario, fluorescence microscopy has been proved as the tool of choice for in vivo recording of brain activity. Optical advances combined with genetically encoded indicators allow a large flexibility in terms of spatiotemporal resolution and field of view while keeping invasiveness in living animals to a minimum. Here we describe the latest advancements in the field of linear and nonlinear optical microscopy with special attention to the exploration of brain functionality of model animals. The present review aims to guide the reader through the main optical systems in the field toward future directions for in vivo microscopy applications.

Sancataldo, G., Silvestri, L., Allegra Mascaro, A.L., Sacconi, L., & Pavone, F.S. (2019). Advanced fluorescence microscopy for in vivo imaging of neuronal activity. OPTICA, 6(6), 758-765 [10.1364/OPTICA.6.000758].

Advanced fluorescence microscopy for in vivo imaging of neuronal activity

Sancataldo, Giuseppe;Silvestri, Ludovico;Pavone, Francesco Saverio
2019

Abstract

Brain function emerges from the coordinated activity, over time, of large neuronal populations placed in different brain regions. Understanding the relationships of these specific areas and disentangling the contributions of individual neurons to overall function remain central goals for neuroscience. In this scenario, fluorescence microscopy has been proved as the tool of choice for in vivo recording of brain activity. Optical advances combined with genetically encoded indicators allow a large flexibility in terms of spatiotemporal resolution and field of view while keeping invasiveness in living animals to a minimum. Here we describe the latest advancements in the field of linear and nonlinear optical microscopy with special attention to the exploration of brain functionality of model animals. The present review aims to guide the reader through the main optical systems in the field toward future directions for in vivo microscopy applications.
Sancataldo, G., Silvestri, L., Allegra Mascaro, A.L., Sacconi, L., & Pavone, F.S. (2019). Advanced fluorescence microscopy for in vivo imaging of neuronal activity. OPTICA, 6(6), 758-765 [10.1364/OPTICA.6.000758].
File in questo prodotto:
File Dimensione Formato  
05_p12_Sancataldo2019_optica.pdf

accesso aperto

Tipologia: Versione Editoriale
Dimensione 1.15 MB
Formato Adobe PDF
1.15 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/468628
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 21
  • ???jsp.display-item.citation.isi??? 21
social impact