The PlTα2 is a sea urchin Paracentrotus lividus gene coding for a neural isotype of alfa tubulin. Expression of Tα2 gene is temporally activated from the blastula stage and spatially confined to the neural territory of the embryo. In order to investigate cis-regulatory elements that control this spatio-temporal pattern we performed functional experiment microinjecting several constructs containing different portion of Tα2 promoter. Moreover quantitative chromatin immune-precipitation experiments were performed to evaluate a possible involvement of chromatin modifications in regulation of Tα2 gene expression.
Nicosia, A., Emanuele, M., Ragusa, M.A., Costa, S. (2009). Transcriptional regulation of Tubulin α2 gene in Paracentrotus lividus.. In V. Cavalieri, G. Morici (a cura di), Excerpts from DBCS. Atti del 6º congresso del dipartimento di biologia cellulare e dello sviluppo «A. Monroy>> (pp. 16-18). Università degli Studi di Palermo.
Transcriptional regulation of Tubulin α2 gene in Paracentrotus lividus.
NICOSIA, Aldo;EMANUELE, Marco;RAGUSA, Maria Antonietta;COSTA, Salvatore
2009-01-01
Abstract
The PlTα2 is a sea urchin Paracentrotus lividus gene coding for a neural isotype of alfa tubulin. Expression of Tα2 gene is temporally activated from the blastula stage and spatially confined to the neural territory of the embryo. In order to investigate cis-regulatory elements that control this spatio-temporal pattern we performed functional experiment microinjecting several constructs containing different portion of Tα2 promoter. Moreover quantitative chromatin immune-precipitation experiments were performed to evaluate a possible involvement of chromatin modifications in regulation of Tα2 gene expression.
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