The catechins derived from green tea possess antioxidant activity and may have a potentially anticancer effect. PTP1B is tyrosine phosphatase that is oxidative stress regulated and is involved with prooncogenic pathways leading to the formation of a.o. breast cancer. Here, we present the effect of selected green tea catechins on enzymatic activity of PTP1B phosphatase and viability of MCF-7 breast cancer cells. We showed also the computational analysis of the most effective catechin binding with a PTP1B molecule. We observed that epigallocatechin, epigallocatechin gallate, epicatechin, and epicatechin gallate may decrease enzymatic activity of PTP1B phosphatase and viability of MCF-7 cells. Conclusions: From the tested compounds, epigallocatechin and epigallocatechin gallate were the most effective inhibitors of the MCF-7 cell viability. Moreover, epigallocatechin was also the strongest inhibitor of PTP1B activity. Computational analysis allows us also to conclude that epigallocatechin is able to interact and bind to PTP1B. Our results suggest also the most predicted binding site to epigallocatechin binding to PTP1B.

Kuban-Jankowska A., Kostrzewa T., Musial C., Barone G., Lo Bosco G., Lo Celso F., et al. (2020). Green tea catechins induce inhibition of ptp1b phosphatase in breast cancer cells with potent anti-cancer properties: In vitro assay, molecular docking, and dynamics studies. ANTIOXIDANTS, 9(12), 1-12 [10.3390/antiox9121208].

Green tea catechins induce inhibition of ptp1b phosphatase in breast cancer cells with potent anti-cancer properties: In vitro assay, molecular docking, and dynamics studies

Barone G.;Lo Bosco G.;Lo Celso F.;
2020

Abstract

The catechins derived from green tea possess antioxidant activity and may have a potentially anticancer effect. PTP1B is tyrosine phosphatase that is oxidative stress regulated and is involved with prooncogenic pathways leading to the formation of a.o. breast cancer. Here, we present the effect of selected green tea catechins on enzymatic activity of PTP1B phosphatase and viability of MCF-7 breast cancer cells. We showed also the computational analysis of the most effective catechin binding with a PTP1B molecule. We observed that epigallocatechin, epigallocatechin gallate, epicatechin, and epicatechin gallate may decrease enzymatic activity of PTP1B phosphatase and viability of MCF-7 cells. Conclusions: From the tested compounds, epigallocatechin and epigallocatechin gallate were the most effective inhibitors of the MCF-7 cell viability. Moreover, epigallocatechin was also the strongest inhibitor of PTP1B activity. Computational analysis allows us also to conclude that epigallocatechin is able to interact and bind to PTP1B. Our results suggest also the most predicted binding site to epigallocatechin binding to PTP1B.
Settore CHIM/03 - Chimica Generale E Inorganica
Kuban-Jankowska A., Kostrzewa T., Musial C., Barone G., Lo Bosco G., Lo Celso F., et al. (2020). Green tea catechins induce inhibition of ptp1b phosphatase in breast cancer cells with potent anti-cancer properties: In vitro assay, molecular docking, and dynamics studies. ANTIOXIDANTS, 9(12), 1-12 [10.3390/antiox9121208].
File in questo prodotto:
File Dimensione Formato  
antioxidants-09-01208-v2.pdf

accesso aperto

Descrizione: articolo principale
Tipologia: Versione Editoriale
Dimensione 1.88 MB
Formato Adobe PDF
1.88 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/454474
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 12
social impact