The present work was designed to identify and characterize novel antimicrobial peptides (AMPs) from Charybdis pancration (Steinh.) Speta, previously named Urginea maritima, is a Mediterranean plant, well-known for its biological properties in traditional medicine. Polypeptide-enriched extracts from different parts of the plant (roots, leaves and bulb), never studied before, were tested against two relevant pathogens, Staphylococcus aureus and Pseudomonas aeruginosa. With the aim of identifying novel natural AMPs, peptide fraction displaying antimicrobial activity (the bulb) that showed minimum inhibitory concentration (MICs) equal to 30 µg/mL against the above mentioned strains, was analysed by high-resolution mass spectrometry and database search. Seventeen peptides, related to seven proteins present in the investigated database, were described. Furthermore, we focused on three peptides, which due to their net positive charge, have a better chance to be AMPs and they were investigated by molecular modelling approaches, in order to shed light on the solution properties of their equilibrium structures. Some of new detected peptides could represent a good platform for the development of new antimicrobials in the fight against antibiotic resistance phenomenon.

Cunsolo V., Schicchi R., Chiaramonte M., Inguglia L., Arizza V., Cusimano M.G., et al. (2020). Identification of new antimicrobial peptides from mediterranean medical plant charybdis pancration (Steinh.) speta. ANTIBIOTICS, 9(11), 1-13 [10.3390/antibiotics9110747].

Identification of new antimicrobial peptides from mediterranean medical plant charybdis pancration (Steinh.) speta

Schicchi R.;Chiaramonte M.;Inguglia L.;Arizza V.;Cusimano M. G.;Schillaci D.
;
Di Francesco A.;Lo Celso F.;Barone G.;
2020-10-28

Abstract

The present work was designed to identify and characterize novel antimicrobial peptides (AMPs) from Charybdis pancration (Steinh.) Speta, previously named Urginea maritima, is a Mediterranean plant, well-known for its biological properties in traditional medicine. Polypeptide-enriched extracts from different parts of the plant (roots, leaves and bulb), never studied before, were tested against two relevant pathogens, Staphylococcus aureus and Pseudomonas aeruginosa. With the aim of identifying novel natural AMPs, peptide fraction displaying antimicrobial activity (the bulb) that showed minimum inhibitory concentration (MICs) equal to 30 µg/mL against the above mentioned strains, was analysed by high-resolution mass spectrometry and database search. Seventeen peptides, related to seven proteins present in the investigated database, were described. Furthermore, we focused on three peptides, which due to their net positive charge, have a better chance to be AMPs and they were investigated by molecular modelling approaches, in order to shed light on the solution properties of their equilibrium structures. Some of new detected peptides could represent a good platform for the development of new antimicrobials in the fight against antibiotic resistance phenomenon.
Settore BIO/02 - Botanica Sistematica
Settore BIO/05 - Zoologia
Settore BIO/19 - Microbiologia Generale
https://www.mdpi.com/2079-6382/9/11/747
Cunsolo V., Schicchi R., Chiaramonte M., Inguglia L., Arizza V., Cusimano M.G., et al. (2020). Identification of new antimicrobial peptides from mediterranean medical plant charybdis pancration (Steinh.) speta. ANTIBIOTICS, 9(11), 1-13 [10.3390/antibiotics9110747].
File in questo prodotto:
File Dimensione Formato  
antibiotics-09-00747-1.pdf

accesso aperto

Descrizione: Articolo principale
Tipologia: Versione Editoriale
Dimensione 2.6 MB
Formato Adobe PDF
2.6 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/438797
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 3
social impact