Thioflavin T (ThT) is a worldwide used dye to monitor protein aggregation as it stains with a certain specificity amyloid structures. The interactions between ThT and its hosts are largely studied suggesting that fluorescence properties of this dye critically depend both on the environment rigidity, electrostatic and hydrophobic properties as well as on molecular details binding site structure. Here FLIM and phasor approach analysis are used to exploit ThT amyloid interactions and, in turn, to address polymorphism and structural heterogeneity of amyloid species mapping aggregate-to-aggregate structural differences and revealing details of molecular architecture within the same aggregate.

Giuseppe Sancataldo; Dirk Fennema Galparsoro;Sara Anselmo; Maurizio Leone; Valeria Vetri (14-18/09/2020).Phasor FLIM analysis of Thioflavin T fluorescence in protein amyloid aggregates: Mapping molecular interactions..

Phasor FLIM analysis of Thioflavin T fluorescence in protein amyloid aggregates: Mapping molecular interactions.

Giuseppe Sancataldo
;
Dirk Fennema Galparsoro;Sara Anselmo;Maurizio Leone;Valeria Vetri

Abstract

Thioflavin T (ThT) is a worldwide used dye to monitor protein aggregation as it stains with a certain specificity amyloid structures. The interactions between ThT and its hosts are largely studied suggesting that fluorescence properties of this dye critically depend both on the environment rigidity, electrostatic and hydrophobic properties as well as on molecular details binding site structure. Here FLIM and phasor approach analysis are used to exploit ThT amyloid interactions and, in turn, to address polymorphism and structural heterogeneity of amyloid species mapping aggregate-to-aggregate structural differences and revealing details of molecular architecture within the same aggregate.
Thioflavin T; FLIM; phasor, amyloid; protein aggregates;
Giuseppe Sancataldo; Dirk Fennema Galparsoro;Sara Anselmo; Maurizio Leone; Valeria Vetri (14-18/09/2020).Phasor FLIM analysis of Thioflavin T fluorescence in protein amyloid aggregates: Mapping molecular interactions..
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/434924
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