The αβ and γδ T cell responses were analyzed in the peripheral blood of children affected by active tuberculosis (TB) and in healthy children who tested positive (PPD+) or negative (PPD-) for purified protein derivative. PPD+ healthy and diseased children responded equally well to PPD in vitro. In contrast, only 18% of PPD+ TB patients responded to peptide p38G derived from the 38-kDa protein of Mycobacterium tuberculosis. Analysis of the whole γδ T cell population and of its Vγ9/Vδ2 subset showed similar frequencies in PPD+ children with TB and in healthy PPD+ and PPD- children. Vγ9/Vδ2 cells from children with TB responded to 5 different phosphoantigens similarly to those from healthy PPD+ children, but healthy PPD- children responded very poorly. Chemotherapy had contrasting effects on the tested lymphocyte population, represented by increase of αβ and decline of Vγ9/Vδ2 T cell responses. T cell responses in childhood TB may be similar to those in adult TB.

Dieli F., Sireci G., Di Sano C., Romano A., Titone L., Di Carlo P., et al. (2000). Ligand-specific αβ and γδ T cell responses in childhood tuberculosis. THE JOURNAL OF INFECTIOUS DISEASES, 181(1), 294-301 [10.1086/315180].

Ligand-specific αβ and γδ T cell responses in childhood tuberculosis

Dieli F.
;
Di Sano C.;Di Carlo P.;Ivanyi J.;
2000

Abstract

The αβ and γδ T cell responses were analyzed in the peripheral blood of children affected by active tuberculosis (TB) and in healthy children who tested positive (PPD+) or negative (PPD-) for purified protein derivative. PPD+ healthy and diseased children responded equally well to PPD in vitro. In contrast, only 18% of PPD+ TB patients responded to peptide p38G derived from the 38-kDa protein of Mycobacterium tuberculosis. Analysis of the whole γδ T cell population and of its Vγ9/Vδ2 subset showed similar frequencies in PPD+ children with TB and in healthy PPD+ and PPD- children. Vγ9/Vδ2 cells from children with TB responded to 5 different phosphoantigens similarly to those from healthy PPD+ children, but healthy PPD- children responded very poorly. Chemotherapy had contrasting effects on the tested lymphocyte population, represented by increase of αβ and decline of Vγ9/Vδ2 T cell responses. T cell responses in childhood TB may be similar to those in adult TB.
Dieli F., Sireci G., Di Sano C., Romano A., Titone L., Di Carlo P., et al. (2000). Ligand-specific αβ and γδ T cell responses in childhood tuberculosis. THE JOURNAL OF INFECTIOUS DISEASES, 181(1), 294-301 [10.1086/315180].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/433561
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