Archivio istituzionale della ricerca dell'Università degli Studi di Palermo
IRIS è il sistema di gestione integrata dei dati della ricerca (persone, pubblicazioni, progetti, attività) adottato dall’Università di Palermo, e ha lo scopo di raccogliere, conservare, documentare, e diffondere ad accesso aperto le informazioni relative alla produzione scientifica degli autori afferenti all’Ateneo.
EnglishFourier transform infrared (FTIR) spectroscopy has been widely used for the analysis of both protein and nucleic acid secondary structure. This is one of the vibration spectroscopy methods that are extremely sensitive to any change in molecular structure. While numerous reports describe how to proceed to analyze protein and deoxyribonucleic acid (DNA) structures using FTIR, reports related to the analyses of ribonucleic acids (RNAs) are few. Nevertheless, RNAs are versatile molecules involved in a multitude of roles in the cell. In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA. The effect of temperature pretreatment, which has a great impact on RNA folding, will also be discussed. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
Geinguenaud F, Militello V, & Arluison V (2020). Application of FTIR Spectroscopy to Analyze RNA Structure. In Methods in Molecular Biology (pp. 119-133).
| Data di pubblicazione: | 2020 |
| Titolo: | Application of FTIR Spectroscopy to Analyze RNA Structure |
| Autori: | |
| Citazione: | Geinguenaud F, Militello V, & Arluison V (2020). Application of FTIR Spectroscopy to Analyze RNA Structure. In Methods in Molecular Biology (pp. 119-133). |
| Abstract: | Fourier transform infrared (FTIR) spectroscopy has been widely used for the analysis of both protein and nucleic acid secondary structure. This is one of the vibration spectroscopy methods that are extremely sensitive to any change in molecular structure. While numerous reports describe how to proceed to analyze protein and deoxyribonucleic acid (DNA) structures using FTIR, reports related to the analyses of ribonucleic acids (RNAs) are few. Nevertheless, RNAs are versatile molecules involved in a multitude of roles in the cell. In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA. The effect of temperature pretreatment, which has a great impact on RNA folding, will also be discussed. © 2020, Springer Science+Business Media, LLC, part of Springer Nature. |
| Appare nelle tipologie: | 2.01 Capitolo o Saggio |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| veronique 2020_10_Online PDF.PDF | Versione Editoriale | Administrator Richiedi una copia |
Copyright © 2020