A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].
ARISTA S, VIZZI E, ALAIMO C, PALERMO D, A. CASCIO (1999). Identification of human rotavirus strains with the P[14] genotype by PCR. JOURNAL OF CLINICAL MICROBIOLOGY, 37, 2706-2708.
Identification of human rotavirus strains with the P[14] genotype by PCR
A. CASCIO
1999-01-01
Abstract
A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].
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