Injectable, in-situ forming kefiran gels have been developed for potential applications as implantable drug delivery devices or scaffolds for tissue regeneration. Concentrated solutions (4, 5 and 6%w) of kefiran, extracted from kefir grains, have been assessed in term of viscosity and injectability through G26 syringe needles, and for their ability to undergo gelation upon mixing with different alcohols. Propylene glycol (PG) has been selected as gelling agent because it ensures homogenous gelation in relatively short times (from few minutes up to 6 h). The investigation of the rheological behavior of kefiran/PG gels varying polymer concentration and temperature (25 °C and 37 °C) has provided interesting hints to support a possible gelation mechanism that accounts also for the observed influence of the alcohol type. Finally, the study of kefiran/PG gels has been complemented with the investigation on selected formulations of the swelling/degradation behavior upon immersion in isotonic buffer solution for up to 40 days at 37 °C; of the ability of the gels to retain and/or release two model molecules; and within vitro cell viability and cytotoxicity tests, to support the absence of toxic effects on cells induced by direct contact with the gels or by leached components from these gels.

Sabatino M.A., Carfi Pavia F., Rigogliuso S., Giacomazza D., Ghersi G., La Carrubba V., et al. (2020). Development of injectable and durable kefiran hydro-alcoholic gels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 149, 309-319 [10.1016/j.ijbiomac.2020.01.244].

Development of injectable and durable kefiran hydro-alcoholic gels

Sabatino M. A.;Carfi Pavia F.;Rigogliuso S.;Ghersi G.;La Carrubba V.;Dispenza C.
2020-01-01

Abstract

Injectable, in-situ forming kefiran gels have been developed for potential applications as implantable drug delivery devices or scaffolds for tissue regeneration. Concentrated solutions (4, 5 and 6%w) of kefiran, extracted from kefir grains, have been assessed in term of viscosity and injectability through G26 syringe needles, and for their ability to undergo gelation upon mixing with different alcohols. Propylene glycol (PG) has been selected as gelling agent because it ensures homogenous gelation in relatively short times (from few minutes up to 6 h). The investigation of the rheological behavior of kefiran/PG gels varying polymer concentration and temperature (25 °C and 37 °C) has provided interesting hints to support a possible gelation mechanism that accounts also for the observed influence of the alcohol type. Finally, the study of kefiran/PG gels has been complemented with the investigation on selected formulations of the swelling/degradation behavior upon immersion in isotonic buffer solution for up to 40 days at 37 °C; of the ability of the gels to retain and/or release two model molecules; and within vitro cell viability and cytotoxicity tests, to support the absence of toxic effects on cells induced by direct contact with the gels or by leached components from these gels.
Settore CHIM/07 - Fondamenti Chimici Delle Tecnologie
Sabatino M.A., Carfi Pavia F., Rigogliuso S., Giacomazza D., Ghersi G., La Carrubba V., et al. (2020). Development of injectable and durable kefiran hydro-alcoholic gels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 149, 309-319 [10.1016/j.ijbiomac.2020.01.244].
File in questo prodotto:
File Dimensione Formato  
Post-print 10.1016j.ijbiomac.2020.01.244.pdf

Open Access dal 26/01/2021

Tipologia: Post-print
Dimensione 3.79 MB
Formato Adobe PDF
3.79 MB Adobe PDF Visualizza/Apri
1-s2.0-S0141813019333707-main.pdf

Solo gestori archvio

Descrizione: pdf
Tipologia: Versione Editoriale
Dimensione 2.18 MB
Formato Adobe PDF
2.18 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/407030
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 5
social impact