Objective: To assess the usefulness of the κ free light chain index (κFLCi) as a screening test to identify patients with suspected MS. Methods: The study included 56 patients with a request to test for oligoclonal bands (OCBs). OCBs were detected by isoelectric focusing, followed by immunofixation. Cerebrospinal fluid (CSF) and serum κFLC were measured by a turbidimetric assay. Also, the κFLC index (κFLCi) was calculated. Results: CSF κFLC levels and κFLCi were significantly higher in patients with multiple sclerosis (MS) than in patients with other neurological diseases (NDs; P < .001 and P < .001, respectively). At the cutoff value of 2.9, the κFLCi detected MS with sensitivity of 97% and specificity of 65%. Overall, 92% patients with κFLCi of 2.9 or greater and who had tested positive for OCBs were diagnosed as having MS. Conclusion: Our findings support the use of κFLCi as a screening test when MS is suspected, followed by OCB detection as a confirmatory test
Agnello, L., Lo Sasso, B., Salemi, G., Altavilla, P., Pappalardo, E.M., Caldarella, R., et al. (2020). Clinical Use of κ Free Light Chains Index as a Screening Test for Multiple Sclerosis. LABORATORY MEDICINE, 51(4), 402-407 [10.1093/labmed/lmz073].
Clinical Use of κ Free Light Chains Index as a Screening Test for Multiple Sclerosis
Agnello, Luisa;Lo Sasso, Bruna;Salemi, Giuseppe;Pappalardo, Emanuela Maria;Caldarella, Rosalia;Meli, Francesco;Scazzone, Concetta;Bivona, Giulia;Ciaccio, Marcello
2020-07-08
Abstract
Objective: To assess the usefulness of the κ free light chain index (κFLCi) as a screening test to identify patients with suspected MS. Methods: The study included 56 patients with a request to test for oligoclonal bands (OCBs). OCBs were detected by isoelectric focusing, followed by immunofixation. Cerebrospinal fluid (CSF) and serum κFLC were measured by a turbidimetric assay. Also, the κFLC index (κFLCi) was calculated. Results: CSF κFLC levels and κFLCi were significantly higher in patients with multiple sclerosis (MS) than in patients with other neurological diseases (NDs; P < .001 and P < .001, respectively). At the cutoff value of 2.9, the κFLCi detected MS with sensitivity of 97% and specificity of 65%. Overall, 92% patients with κFLCi of 2.9 or greater and who had tested positive for OCBs were diagnosed as having MS. Conclusion: Our findings support the use of κFLCi as a screening test when MS is suspected, followed by OCB detection as a confirmatory testFile | Dimensione | Formato | |
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