CENP-A is the histone H3 variant necessary to specify the location of all eukaryotic centromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7, has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. Thus, we conclude that phosphorylation of CENP-A on serine 7 is dispensable to maintain correct centromere dynamics and function.

Barra V., Logsdon G.A., Scelfo A., Hoffmann S., Herve S., Aslanian A., et al. (2019). Phosphorylation of CENP-A on serine 7 does not control centromere function. NATURE COMMUNICATIONS, 10(1), 1-10 [10.1038/s41467-018-08073-1].

Phosphorylation of CENP-A on serine 7 does not control centromere function

Barra V.;
2019-12-01

Abstract

CENP-A is the histone H3 variant necessary to specify the location of all eukaryotic centromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7, has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. Thus, we conclude that phosphorylation of CENP-A on serine 7 is dispensable to maintain correct centromere dynamics and function.
Settore BIO/18 - Genetica
https://www.nature.com/articles/s41467-018-08073-1
Barra V., Logsdon G.A., Scelfo A., Hoffmann S., Herve S., Aslanian A., et al. (2019). Phosphorylation of CENP-A on serine 7 does not control centromere function. NATURE COMMUNICATIONS, 10(1), 1-10 [10.1038/s41467-018-08073-1].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/391406
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