The administration of cryopreserved platelets (PLTs) may overcome the limits of platelet shortage and availability, especially during some seasons or in specific contexts like rural areas. After in vitro validation studies, ad hoc prepared buffy coat-derived pooled platelet concentrates (BC-PLTs), treated with dimethyl sulphoxide (DMSO) and cryopreserved (CRY BC-PLTs) at -80 °C with a modified Valeri method, were transfused in patients with severe thrombocytopenia secondary to chemotherapy for acute leukaemia (AL). Five inpatients were enrolled in the pivotal clinical trial NCT02032134: 4 males and 1 female with a mean age of 71 years (range: 65–80). Four patients were diagnosed with acute myeloid leukaemia and 1 had acute lymphoblastic leukaemia.Transfusion of one Unit of CRY BC-PLTs resulted effective in active bleeding control in two patients without any adverse reaction or concomitant antihaemorrhagic therapies. CRY BC-PLTs met the currently accepted criteria for cryopreserved PLTs, their transfusion in patients with AL was safe. (Clinical trial: NCT02032134)

Napolitano, M., Mancuso, S., Raso, S., LoCoco, L., Arfò, P.S., De Francisci, G., et al. (2019). Buffy coat-derived platelets cryopreserved using a new method: Results from a pivotal clinical trial on thrombocytopenic patients with acute leukaemia. TRANSFUSION AND APHERESIS SCIENCE, 58(6) [10.1016/j.transci.2019.10.008].

Buffy coat-derived platelets cryopreserved using a new method: Results from a pivotal clinical trial on thrombocytopenic patients with acute leukaemia

Napolitano, Mariasanta
Conceptualization
;
Mancuso, Salvatrice
Investigation
;
Raso, Simona
Investigation
;
Arfò, Piera Stefania
Methodology
;
Dieli, Francesco
Data Curation
;
Caccamo, Nadia
Investigation
;
Dolce, Alberto
Formal Analysis
;
Agliastro, Rosalia
Membro del Collaboration Group
;
Siragusa, Sergio
Writing – Review & Editing
2019-01-01

Abstract

The administration of cryopreserved platelets (PLTs) may overcome the limits of platelet shortage and availability, especially during some seasons or in specific contexts like rural areas. After in vitro validation studies, ad hoc prepared buffy coat-derived pooled platelet concentrates (BC-PLTs), treated with dimethyl sulphoxide (DMSO) and cryopreserved (CRY BC-PLTs) at -80 °C with a modified Valeri method, were transfused in patients with severe thrombocytopenia secondary to chemotherapy for acute leukaemia (AL). Five inpatients were enrolled in the pivotal clinical trial NCT02032134: 4 males and 1 female with a mean age of 71 years (range: 65–80). Four patients were diagnosed with acute myeloid leukaemia and 1 had acute lymphoblastic leukaemia.Transfusion of one Unit of CRY BC-PLTs resulted effective in active bleeding control in two patients without any adverse reaction or concomitant antihaemorrhagic therapies. CRY BC-PLTs met the currently accepted criteria for cryopreserved PLTs, their transfusion in patients with AL was safe. (Clinical trial: NCT02032134)
Napolitano, M., Mancuso, S., Raso, S., LoCoco, L., Arfò, P.S., De Francisci, G., et al. (2019). Buffy coat-derived platelets cryopreserved using a new method: Results from a pivotal clinical trial on thrombocytopenic patients with acute leukaemia. TRANSFUSION AND APHERESIS SCIENCE, 58(6) [10.1016/j.transci.2019.10.008].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/384648
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