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EnglishThis work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged.
| Data di pubblicazione: | 2018 |
| Titolo: | Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization |
| Autori: | |
| Citazione: | Josts, I., Niebling, S., Gao, Y., Levantino, M., Tidow, H., & Monteiro, D. (2018). Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization. IUCRJ, 5(6), 667-672. |
| Rivista: | |
| Digital Object Identifier (DOI): | 10.1107/S2052252518012149 |
| Abstract: | This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged. |
| URL: | https://journals.iucr.org/m/issues/2018/06/00/mf5027/index.html |
| Settore Scientifico Disciplinare: | Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin) |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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