We report the identification of a cDNA that encodes a putative protein of 94 amino acids and expected molecular weight of 10.7 kDa, the C-terminal half of which is identical to that of PEP19, a small, brain-specific protein involved in Ca++/calmodulin signaling. The novel rat-specific protein, tentatively named long PEP19 isoform (LPI), is the product of alternative splicing of the rat PCP4 gene encoding PEP19. We found that antibodies raised against the first 13 N-terminal amino acids of LPI, not present in PEP19, recognize a protein enriched in the developing rat brain.
SALA, A., SCATURRO, M., PROIA, P., SCHIERA, G., BALISTRERI, E., ALALO-RATTENBACH, R., et al. (2007). CLONING OF RAT-SPECIFIC LONG PCP4/PEP19 ISOFORM (LPI). INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2007, 501-509.
CLONING OF RAT-SPECIFIC LONG PCP4/PEP19 ISOFORM (LPI)
PROIA, Patrizia;SCHIERA, Gabriella;DI LIEGRO, Italia
2007-01-01
Abstract
We report the identification of a cDNA that encodes a putative protein of 94 amino acids and expected molecular weight of 10.7 kDa, the C-terminal half of which is identical to that of PEP19, a small, brain-specific protein involved in Ca++/calmodulin signaling. The novel rat-specific protein, tentatively named long PEP19 isoform (LPI), is the product of alternative splicing of the rat PCP4 gene encoding PEP19. We found that antibodies raised against the first 13 N-terminal amino acids of LPI, not present in PEP19, recognize a protein enriched in the developing rat brain.
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