PTP1B phosphatase as a novel target of oleuropein activity in MCF-7 breast cancer model

Przychodzen, P.; Kuban-Jankowska, A.; Wyszkowska, R.; Barone, G.; Lo Bosco, G.; Lo Celso, F.; Kamm, A.; Daca, A.; Kostrzewa, T.; Gorska-Ponikowska, M.

doi:10.1016/j.tiv.2019.104624

Archivio istituzionale della ricerca dell'Università degli Studi di Palermo

IRIS è il sistema di gestione integrata dei dati della ricerca (persone, pubblicazioni, progetti, attività) adottato dall’Università di Palermo, e ha lo scopo di raccogliere, conservare, documentare, e diffondere ad accesso aperto le informazioni relative alla produzione scientifica degli autori afferenti all’Ateneo.

Phosphatase PTP1B has become a therapeutic target for the treatment of type 2-diabetes, whereas recent studies have revealed that PTP1B plays a pivotal role in pathophysiology and development of breast cancer. Oleuropein is a natural, phenolic compound with anticancer activity. The aim of this study was to address the question whether PTP1B constitutes a target for oleuropein in breast cancer MCF-7 cells. The cellular MCF-7 breast cancer model was used in the study. The experiments were performed using cellular viability tests, Elisa assays, immunoprecipitation, flow cytometry analyses and computer modelling. Herein, we evidenced that the reduced activity of phosphatase PTP1B after treatment with oleuropein is strictly correlated with decreased MCF-7 cellular viability and cell cycle arrest. These results provide new insight into further research on oleuropein and possible role of the compound in adjuvant treatment of breast cancer

Data di pubblicazione:	2019
Titolo:	PTP1B phosphatase as a novel target of oleuropein activity in MCF-7 breast cancer model
Autori:	Przychodzen, Paulina Kuban-Jankowska, Alicja Wyszkowska, Roksana BARONE, Giampaolo LO BOSCO, Giosue' LO CELSO, Fabrizio Kamm, Anna Daca, Agnieszka Kostrzewa, Tomasz Gorska-Ponikowska, Magdalena mostra contributor esterni nascondi contributor esterni
Citazione:	Przychodzen, P., Kuban-Jankowska, A., Wyszkowska, R., Barone, G., Lo Bosco, G., Lo Celso, F., et al. (2019). PTP1B phosphatase as a novel target of oleuropein activity in MCF-7 breast cancer model. TOXICOLOGY IN VITRO, 61(2019), 1-9.
Rivista:	TOXICOLOGY IN VITRO
Digital Object Identifier (DOI):	10.1016/j.tiv.2019.104624
Abstract:	Phosphatase PTP1B has become a therapeutic target for the treatment of type 2-diabetes, whereas recent studies have revealed that PTP1B plays a pivotal role in pathophysiology and development of breast cancer. Oleuropein is a natural, phenolic compound with anticancer activity. The aim of this study was to address the question whether PTP1B constitutes a target for oleuropein in breast cancer MCF-7 cells. The cellular MCF-7 breast cancer model was used in the study. The experiments were performed using cellular viability tests, Elisa assays, immunoprecipitation, flow cytometry analyses and computer modelling. Herein, we evidenced that the reduced activity of phosphatase PTP1B after treatment with oleuropein is strictly correlated with decreased MCF-7 cellular viability and cell cycle arrest. These results provide new insight into further research on oleuropein and possible role of the compound in adjuvant treatment of breast cancer
Settore Scientifico Disciplinare:	Settore CHIM/02 - Chimica Fisica Settore CHIM/03 - Chimica Generale E Inorganica Settore BIO/14 - Farmacologia
Appare nelle tipologie:	1.01 Articolo in rivista

File in questo prodotto:

File	Descrizione	Tipologia	Licenza
1-s2.0-S0887233319304564-main.pdf		Versione Editoriale		Administrator Richiedi una copia

Utilizza questo identificativo per citare o creare un link a questo documento:
http://hdl.handle.net/10447/367184

Citazioni

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.