A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH 4 -Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.

Mannina G., Presti D., Montiel-Jarillo G., Suarez-Ojeda M.E. (2019). Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures. BIORESOURCE TECHNOLOGY, 282, 361-369 [10.1016/j.biortech.2019.03.037].

Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures

Mannina G.
;
2019-01-01

Abstract

A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH 4 -Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.
2019
Settore ICAR/03 - Ingegneria Sanitaria-Ambientale
Mannina G., Presti D., Montiel-Jarillo G., Suarez-Ojeda M.E. (2019). Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures. BIORESOURCE TECHNOLOGY, 282, 361-369 [10.1016/j.biortech.2019.03.037].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/367164
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