We developed a method which allows electrophoretic fractionation of DNA in an agarose matrix according to an increasing current gradient, using a previously designed [R. Barbieri, V. Izzo, M.A. Costa, G. Giudice, G. Duro, Anal. Biochem. 212 (1993) 168; M.R. Asaro, V. Izzo, R. Barbieri, J. Chromatogr. A 855 (1999) 723] voltage gradient apparatus. This method allows the separation of different DNA fragments by increasing the distances of the components fractionated in the gel, revealing small differences in the length of different DNA components.

OLIVA, D., DI MAIO, S., SISINO, G., BELLAVIA, D., BARBIERI, R. (2008). Increasing voltage gradient electrophoresis of DNA. JOURNAL OF CHROMATOGRAPHY A, 1187, 205-207.

Increasing voltage gradient electrophoresis of DNA

Di Maio, Sabina;BELLAVIA, Daniele;BARBIERI, Rainero
2008-01-01

Abstract

We developed a method which allows electrophoretic fractionation of DNA in an agarose matrix according to an increasing current gradient, using a previously designed [R. Barbieri, V. Izzo, M.A. Costa, G. Giudice, G. Duro, Anal. Biochem. 212 (1993) 168; M.R. Asaro, V. Izzo, R. Barbieri, J. Chromatogr. A 855 (1999) 723] voltage gradient apparatus. This method allows the separation of different DNA fragments by increasing the distances of the components fractionated in the gel, revealing small differences in the length of different DNA components.
2008
Settore BIO/18 - Genetica
OLIVA, D., DI MAIO, S., SISINO, G., BELLAVIA, D., BARBIERI, R. (2008). Increasing voltage gradient electrophoresis of DNA. JOURNAL OF CHROMATOGRAPHY A, 1187, 205-207.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/35977
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