The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).

DAMIANO, G., GIOVIALE, M.C., LOMBARDO, C., & LO MONTE, A.I. (2009). An old method for good new cells. TRANSPLANTATION PROCEEDINGS, 41, 1116-1118 [10.1016/j.transproceed.2009.03.078].

An old method for good new cells

DAMIANO, Giuseppe;GIOVIALE, Maria Concetta;LOMBARDO, Calogera;LO MONTE, Attilio Ignazio
2009

Abstract

The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).
Settore MED/18 - Chirurgia Generale
DAMIANO, G., GIOVIALE, M.C., LOMBARDO, C., & LO MONTE, A.I. (2009). An old method for good new cells. TRANSPLANTATION PROCEEDINGS, 41, 1116-1118 [10.1016/j.transproceed.2009.03.078].
File in questo prodotto:
File Dimensione Formato  
An Old Method for Good New Cells.pdf

accesso aperto

Dimensione 866.45 kB
Formato Adobe PDF
866.45 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/35938
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 3
social impact