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EnglishThe aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).
Damiano, G., Gioviale, M., Lombardo, C., & LO MONTE, A. (2009). An old method for good new cells. TRANSPLANTATION PROCEEDINGS, 41, 1116-1118.
| Data di pubblicazione: | 2009 | |
| Titolo: | An old method for good new cells | |
| Autori: | ||
| Citazione: | Damiano, G., Gioviale, M., Lombardo, C., & LO MONTE, A. (2009). An old method for good new cells. TRANSPLANTATION PROCEEDINGS, 41, 1116-1118. | |
| Rivista: | ||
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.transproceed.2009.03.078 | |
| Abstract: | The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square). | |
| Settore Scientifico Disciplinare: | Settore MED/18 - Chirurgia Generale | |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| An Old Method for Good New Cells.pdf | N/A | Open Access Visualizza/Apri |
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