The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).

DAMIANO, G., GIOVIALE, M.C., LOMBARDO, C., LO MONTE, A.I. (2009). An old method for good new cells. TRANSPLANTATION PROCEEDINGS, 41, 1116-1118 [10.1016/j.transproceed.2009.03.078].

An old method for good new cells

DAMIANO, Giuseppe;GIOVIALE, Maria Concetta;LOMBARDO, Calogera;LO MONTE, Attilio Ignazio
2009-01-01

Abstract

The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).
2009
DAMIANO, G., GIOVIALE, M.C., LOMBARDO, C., LO MONTE, A.I. (2009). An old method for good new cells. TRANSPLANTATION PROCEEDINGS, 41, 1116-1118 [10.1016/j.transproceed.2009.03.078].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/35938
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