Till date, the utility of cytologic assessment of ascites for the identification of hepatocellular carcinoma (HCC) cells is still debated and the usefulness of immunocytochemistry for glypican-3 (GPC3) and Hep Par-1 in this setting has not been reported. Liquid-based cytology of ascitic fluid of 28 cirrhotic patients was performed and the spots obtained were stained with hematoxylin and eosin, papanicolau, and with GPC3 and Hep Par-1 antibodies. GPC3 and Hep Par-1 antibodies stained positively the atypical cells in the ascites of 2 patients with HCC showing an exophytic growth pattern. The specimens of the patients with nonexophytic HCC, other non-HCC cancers, or cirrhosis stained negatively. We hypothesize a relationship with the exophytic growth of the tumor. Thus, GPC3 and Hep Par-1 are useful in the cytologic assessment of peritoneal effusions to distinguish mesothelial cells and neoplastic non-HCC cells from HCC cells, presence of which, however, represent a very rare event. This is the first study in which GPC3 and Hep Par-1 immunostaining has been used in the cytologic assessment of HCC ascites.
|Titolo:||Glypican-3 and Hep Par-1 are Useful Biomarkers in the Cytologic Assessment of Ascites|
|Data di pubblicazione:||gen-2019|
|Citazione:||Cabibi, D., Bisanti, A., Lanza, D., Brucculeri, V., Porcasi, R., Ingrao, S., et al. (2019). Glypican-3 and Hep Par-1 are Useful Biomarkers in the Cytologic Assessment of Ascites.|
|Appare nelle tipologie:||1.01 Articolo in rivista|