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EnglishA new fluorescent, non-cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G-quadruplexes with scarce or no affinity towards single-or double-stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G-quad-ruplex structures in solution by use of PDI2B and strepta-vidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G-quadruplex structures from solution, even in the presence of an excess of single-stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2-over 3-tetrad quadruplexes, which constitutes a novel type of behavior.
| Data di pubblicazione: | 2018 |
| Titolo: | Fishing for G-quadruplexes in solution with a perylene diimide derivative labeled with biotins |
| Autori: | |
| Citazione: | Busto, N., Calvo, P., Santolaya, J., Leal, J., Guédin, A., Barone, G., et al. (2018). Fishing for G-quadruplexes in solution with a perylene diimide derivative labeled with biotins. CHEMISTRY-A EUROPEAN JOURNAL, 24(44), 11292-11296. |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/chem.201802365 |
| Abstract: | A new fluorescent, non-cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G-quadruplexes with scarce or no affinity towards single-or double-stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G-quad-ruplex structures in solution by use of PDI2B and strepta-vidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G-quadruplex structures from solution, even in the presence of an excess of single-stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2-over 3-tetrad quadruplexes, which constitutes a novel type of behavior. |
| URL: | http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-3765 |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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