Purpose: Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response. Materials and methods: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses. Results: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of ‘senescence-associated secretory phenotype’ (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process. Conclusions: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.

Bravatà, V., Minafra, L., Forte, G.I., Cammarata, F.P., Russo, G., Di Maggio, F.M., et al. (2017). Cytokine profile of breast cell lines after different radiation doses. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 93(11), 1217-1226 [10.1080/09553002.2017.1362504].

Cytokine profile of breast cell lines after different radiation doses

Bravatà, Valentina
Writing – Review & Editing
;
Minafra, Luigi
Writing – Original Draft Preparation
;
Forte, Giusi Irma
Writing – Review & Editing
;
Augello, Giuseppa
Writing – Review & Editing
;
Lio, Domenico
Writing – Review & Editing
;
2017

Abstract

Purpose: Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response. Materials and methods: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses. Results: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of ‘senescence-associated secretory phenotype’ (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process. Conclusions: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.
https://www.tandfonline.com/doi/abs/10.1080/09553002.2017.1362504?journalCode=irab20
Bravatà, V., Minafra, L., Forte, G.I., Cammarata, F.P., Russo, G., Di Maggio, F.M., et al. (2017). Cytokine profile of breast cell lines after different radiation doses. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 93(11), 1217-1226 [10.1080/09553002.2017.1362504].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/326374
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