Cryopreservation for the long-term storage of platelets (PLTs) is a useful method to overcome the limits of platelet shortage. This is an in vitro prospective study to evaluate the count, viability, and function of buffy coat-derived pooled platelet concentrates (BC-PLTs), treated with dimethyl sulphoxide (DMSO) and cryopreserved (CRY BC-PLTs) at −80 °C with a modified Valeri method. PLTs were stored in 6% DMSO with a patented kit. Overall, 49 BC-PLTs from 245 healthy volunteer donors were prepared, cryopreserved, and analysed before and after 3, 6, and 9 months of storage. In flow cytometry, a statistically significant reduction in CD 42b (92.7 ± 4.29% at T0 vs. 23.6 ± 27.5% at T3, 16.38 ± 12.54% at T6, and 17.3 ± 9.6% at T9) and PAC-1 (1.9 ± 1.34% at T0 vs. 0.62 ± 0.4% at T3, 0.63 ± 0.83% at T6, and 0.49 ± 0.48% at T9) was observed after storage. CRY BC-PLTs showed a good and stable endogenous thrombin generation potential (nM min): 529.25 ± 98.64 at T0 vs. 533.04 ± 103.15 at T9 months. CRY BC-PLTs showed a good viability in vitro, according to currently accepted criteria for cryopreserved PLTs.

Napolitano, M., Mancuso, S., Lo Coco, L., Arfò, P.S., Raso, S., De Francisci, G., et al. (2018). Buffy coat-derived platelets cryopreserved using a new method: Results from in vitro studies. TRANSFUSION AND APHERESIS SCIENCE [10.1016/j.transci.2018.07.020].

Buffy coat-derived platelets cryopreserved using a new method: Results from in vitro studies

Napolitano, Mariasanta
Investigation
;
Mancuso, Salvatrice;Lo Coco, Lucio;ARFO', Piera Stefania;RASO, Simona;Dieli, Francesco;Caccamo, Nadia;Dolce, Alberto;Agliastro, Rosalia;Siragusa, Sergio
2018

Abstract

Cryopreservation for the long-term storage of platelets (PLTs) is a useful method to overcome the limits of platelet shortage. This is an in vitro prospective study to evaluate the count, viability, and function of buffy coat-derived pooled platelet concentrates (BC-PLTs), treated with dimethyl sulphoxide (DMSO) and cryopreserved (CRY BC-PLTs) at −80 °C with a modified Valeri method. PLTs were stored in 6% DMSO with a patented kit. Overall, 49 BC-PLTs from 245 healthy volunteer donors were prepared, cryopreserved, and analysed before and after 3, 6, and 9 months of storage. In flow cytometry, a statistically significant reduction in CD 42b (92.7 ± 4.29% at T0 vs. 23.6 ± 27.5% at T3, 16.38 ± 12.54% at T6, and 17.3 ± 9.6% at T9) and PAC-1 (1.9 ± 1.34% at T0 vs. 0.62 ± 0.4% at T3, 0.63 ± 0.83% at T6, and 0.49 ± 0.48% at T9) was observed after storage. CRY BC-PLTs showed a good and stable endogenous thrombin generation potential (nM min): 529.25 ± 98.64 at T0 vs. 533.04 ± 103.15 at T9 months. CRY BC-PLTs showed a good viability in vitro, according to currently accepted criteria for cryopreserved PLTs.
www.elsevier.com/locate/transci
Napolitano, M., Mancuso, S., Lo Coco, L., Arfò, P.S., Raso, S., De Francisci, G., et al. (2018). Buffy coat-derived platelets cryopreserved using a new method: Results from in vitro studies. TRANSFUSION AND APHERESIS SCIENCE [10.1016/j.transci.2018.07.020].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/305592
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