In this paper, new composite nanoparticles based on hyaluronic acid (HA) chemically cross-linked with alpha,beta polyaspartylhydrazide (PAHy) were prepared by the use of a reversed-phase microemulsion technique. HA-PAHy nanoparticles were characterized by FT-IR spectroscopy, confirming the occurrence of the chemical cross-linking, dimensional analysis, and transmission electron micrography, showing a sub-micrometer size and spherical shape. Zeta potential measurements demonstrated the presence of HA on the nanoparticle surface. A remarkable affinity of the obtained nanoparticles toward aqueous media that simulate some biological fluids was found. Stability studies showed the absence of chemical degradation in various media, while in the presence of hyaluronidase, a partial degradation occurred. Cell compatibility was evaluated by performing in vitro assays on human chronic myelogenous leukaemia cells (K-562) chosen as a model cell line and a haemolytic test. HA PAHy nanoparticles were also able to entrap 5-fluorouracil, chosen as a model drug, and release it in a simulated physiological fluid and in human plasma with a mechanism essentially controlled by a Fickian diffusion.

PITARRESI, G., CRAPARO, E.F., PALUMBO, F.S., CARLISI, B., GIAMMONA, G. (2007). COMPOSITE NANOPARTICLES BASED ON HYALURONIC ACID CHEMICALLY CROSS-LINKED WITH α,β-POLYASPARTYLHYDRAZIDE. BIOMACROMOLECULES, 8 (6), 1890-1898 [10.1021/bm070224a].

COMPOSITE NANOPARTICLES BASED ON HYALURONIC ACID CHEMICALLY CROSS-LINKED WITH α,β-POLYASPARTYLHYDRAZIDE

PITARRESI, Giovanna;CRAPARO, Emanuela Fabiola;PALUMBO, Fabio Salvatore;CARLISI, Bianca Maria;GIAMMONA, Gaetano
2007-01-01

Abstract

In this paper, new composite nanoparticles based on hyaluronic acid (HA) chemically cross-linked with alpha,beta polyaspartylhydrazide (PAHy) were prepared by the use of a reversed-phase microemulsion technique. HA-PAHy nanoparticles were characterized by FT-IR spectroscopy, confirming the occurrence of the chemical cross-linking, dimensional analysis, and transmission electron micrography, showing a sub-micrometer size and spherical shape. Zeta potential measurements demonstrated the presence of HA on the nanoparticle surface. A remarkable affinity of the obtained nanoparticles toward aqueous media that simulate some biological fluids was found. Stability studies showed the absence of chemical degradation in various media, while in the presence of hyaluronidase, a partial degradation occurred. Cell compatibility was evaluated by performing in vitro assays on human chronic myelogenous leukaemia cells (K-562) chosen as a model cell line and a haemolytic test. HA PAHy nanoparticles were also able to entrap 5-fluorouracil, chosen as a model drug, and release it in a simulated physiological fluid and in human plasma with a mechanism essentially controlled by a Fickian diffusion.
2007
PITARRESI, G., CRAPARO, E.F., PALUMBO, F.S., CARLISI, B., GIAMMONA, G. (2007). COMPOSITE NANOPARTICLES BASED ON HYALURONIC ACID CHEMICALLY CROSS-LINKED WITH α,β-POLYASPARTYLHYDRAZIDE. BIOMACROMOLECULES, 8 (6), 1890-1898 [10.1021/bm070224a].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/29519
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 36
social impact