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EnglishThe immune system of marine invertebrates, in particular that of holothurians, still requires further study. Our research showed that coelomocyte cells contained in the coelomic fluid of the sea cucumber, Holothuria tubulosa, are able to lyse, in vitro, red blood cells in rabbits and sheep. A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions of each pattern were resolved on a polyacrylamide gel and calcium-dependent and independent coelomocyte lysate patterns were compared.
Vazzana, M., Celi, M., Chiaramonte, M., Inguglia, L., Russo, D., Ferrantelli, V., et al. (2018). Cytotoxic activity of Holothuria tubulosa (Echinodermata) coelomocytes. FISH AND SHELLFISH IMMUNOLOGY, 72, 334-341.
| Data di pubblicazione: | 2018 |
| Titolo: | Cytotoxic activity of Holothuria tubulosa (Echinodermata) coelomocytes |
| Autori: | ARIZZA, Vincenzo (Corresponding) |
| Citazione: | Vazzana, M., Celi, M., Chiaramonte, M., Inguglia, L., Russo, D., Ferrantelli, V., et al. (2018). Cytotoxic activity of Holothuria tubulosa (Echinodermata) coelomocytes. FISH AND SHELLFISH IMMUNOLOGY, 72, 334-341. |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.fsi.2017.11.021 |
| Abstract: | The immune system of marine invertebrates, in particular that of holothurians, still requires further study. Our research showed that coelomocyte cells contained in the coelomic fluid of the sea cucumber, Holothuria tubulosa, are able to lyse, in vitro, red blood cells in rabbits and sheep. A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions of each pattern were resolved on a polyacrylamide gel and calcium-dependent and independent coelomocyte lysate patterns were compared. |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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