Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts the latter.
|Data di pubblicazione:||2007|
|Titolo:||Mid-region PTHrP and gene expression of MDA-MB231 breast cancer cells|
|Autori:||SIRCHIA R; LUPARELLO C|
|Tipologia:||Articolo su rivista|
|Citazione:||SIRCHIA R, & LUPARELLO C (2007). Mid-region PTHrP and gene expression of MDA-MB231 breast cancer cells. BIOLOGICAL CHEMISTRY, 388, 457-465.|
|Digital Object Identifier (DOI):||10.1515/BC.2007.059|
|Appare nelle tipologie:||01 - Articolo su rivista|