Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts the latter.

Sirchia, R., Luparello, C. (2007). Mid-region PTHrP and gene expression of MDA-MB231 breast cancer cells. BIOLOGICAL CHEMISTRY, 388(388), 457-465 [10.1515/BC.2007.059].

Mid-region PTHrP and gene expression of MDA-MB231 breast cancer cells

LUPARELLO, Claudio
2007-01-01

Abstract

Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts the latter.
2007
Settore BIO/06 - Anatomia Comparata E Citologia
Sirchia, R., Luparello, C. (2007). Mid-region PTHrP and gene expression of MDA-MB231 breast cancer cells. BIOLOGICAL CHEMISTRY, 388(388), 457-465 [10.1515/BC.2007.059].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/25501
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