Objective and design: Epigenetic regulation is important in the activation of inflammatory cells. In the present study, we evaluated if DNA-methylation variations are involved in Interleukin-1β (IL-1β)-induced intestinal epithelial cells activation. Materials and methods: Differentiated Caco-2 cells were exposed to IL-1β or to 5-azadeoxycytidine (5-azadC) for 24 or 48 h. Genome-wide methylation status was evaluated, while DNA methylation status at the promoter region of the gene encoding interleukin-6, 8 and 10 (IL-6, 8 and 10) was estimated. The levels of the corresponding gene products as well as DNA methyltransferases (DNMTs) quantity were assessed. Results: IL-1β decreased genomic methylation of human intestinal epithelial cells and induced demethylation at cg-specific sites at the promoter of pro-inflammatory genes IL6 and IL8; conversely it did not change the methylation of the IL10 promoter. IL-1β also increased the release of IL-6 and IL-8 but did not change the IL-10 expression. Finally, cell exposure to IL-1β decreased the DNMT3b expression, increased DNMT3a and was not able to change DNMT1 expression. Conclusions: Our results suggest a potential role of IL-1β as modulator of DNA methylation in activated differentiated Caco-2 cell line.

Caradonna, F., Cruciata, I., Schifano, I., la Rosa, C., Naselli, F., Chiarelli, R., et al. (2017). Methylation of cytokines gene promoters in IL-1β-treated human intestinal epithelial cells. INFLAMMATION RESEARCH, 67(4), 327-337 [10.1007/s00011-017-1124-5].

Methylation of cytokines gene promoters in IL-1β-treated human intestinal epithelial cells

Caradonna, Fabio
;
Cruciata, Ilenia;SCHIFANO, Ilaria;Naselli, Flores;Chiarelli, Roberto;Perrone, Anna;Gentile, Carla
2017

Abstract

Objective and design: Epigenetic regulation is important in the activation of inflammatory cells. In the present study, we evaluated if DNA-methylation variations are involved in Interleukin-1β (IL-1β)-induced intestinal epithelial cells activation. Materials and methods: Differentiated Caco-2 cells were exposed to IL-1β or to 5-azadeoxycytidine (5-azadC) for 24 or 48 h. Genome-wide methylation status was evaluated, while DNA methylation status at the promoter region of the gene encoding interleukin-6, 8 and 10 (IL-6, 8 and 10) was estimated. The levels of the corresponding gene products as well as DNA methyltransferases (DNMTs) quantity were assessed. Results: IL-1β decreased genomic methylation of human intestinal epithelial cells and induced demethylation at cg-specific sites at the promoter of pro-inflammatory genes IL6 and IL8; conversely it did not change the methylation of the IL10 promoter. IL-1β also increased the release of IL-6 and IL-8 but did not change the IL-10 expression. Finally, cell exposure to IL-1β decreased the DNMT3b expression, increased DNMT3a and was not able to change DNMT1 expression. Conclusions: Our results suggest a potential role of IL-1β as modulator of DNA methylation in activated differentiated Caco-2 cell line.
Settore BIO/18 - Genetica
Settore BIO/10 - Biochimica
Settore BIO/13 - Biologia Applicata
Caradonna, F., Cruciata, I., Schifano, I., la Rosa, C., Naselli, F., Chiarelli, R., et al. (2017). Methylation of cytokines gene promoters in IL-1β-treated human intestinal epithelial cells. INFLAMMATION RESEARCH, 67(4), 327-337 [10.1007/s00011-017-1124-5].
File in questo prodotto:
File Dimensione Formato  
Caradonna et al 2017.pdf

Solo gestori archvio

Descrizione: pdf del'articolo
Dimensione 2.08 MB
Formato Adobe PDF
2.08 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/251235
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 24
social impact