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EnglishObjective and design: Epigenetic regulation is important in the activation of inflammatory cells. In the present study, we evaluated if DNA-methylation variations are involved in Interleukin-1β (IL-1β)-induced intestinal epithelial cells activation. Materials and methods: Differentiated Caco-2 cells were exposed to IL-1β or to 5-azadeoxycytidine (5-azadC) for 24 or 48 h. Genome-wide methylation status was evaluated, while DNA methylation status at the promoter region of the gene encoding interleukin-6, 8 and 10 (IL-6, 8 and 10) was estimated. The levels of the corresponding gene products as well as DNA methyltransferases (DNMTs) quantity were assessed. Results: IL-1β decreased genomic methylation of human intestinal epithelial cells and induced demethylation at cg-specific sites at the promoter of pro-inflammatory genes IL6 and IL8; conversely it did not change the methylation of the IL10 promoter. IL-1β also increased the release of IL-6 and IL-8 but did not change the IL-10 expression. Finally, cell exposure to IL-1β decreased the DNMT3b expression, increased DNMT3a and was not able to change DNMT1 expression. Conclusions: Our results suggest a potential role of IL-1β as modulator of DNA methylation in activated differentiated Caco-2 cell line.
Caradonna, F., Cruciata, I., Schifano, I., la Rosa, C., Naselli, F., Chiarelli, R., et al. (2017). Methylation of cytokines gene promoters in IL-1β-treated human intestinal epithelial cells. INFLAMMATION RESEARCH, 67(4), 327-337 [10.1007/s00011-017-1124-5].
| Data di pubblicazione: | 2017 | |
| Titolo: | Methylation of cytokines gene promoters in IL-1β-treated human intestinal epithelial cells | |
| Autori: | GENTILE, Carla (Corresponding) | |
| Citazione: | Caradonna, F., Cruciata, I., Schifano, I., la Rosa, C., Naselli, F., Chiarelli, R., et al. (2017). Methylation of cytokines gene promoters in IL-1β-treated human intestinal epithelial cells. INFLAMMATION RESEARCH, 67(4), 327-337 [10.1007/s00011-017-1124-5]. | |
| Rivista: | ||
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/s00011-017-1124-5 | |
| Abstract: | Objective and design: Epigenetic regulation is important in the activation of inflammatory cells. In the present study, we evaluated if DNA-methylation variations are involved in Interleukin-1β (IL-1β)-induced intestinal epithelial cells activation. Materials and methods: Differentiated Caco-2 cells were exposed to IL-1β or to 5-azadeoxycytidine (5-azadC) for 24 or 48 h. Genome-wide methylation status was evaluated, while DNA methylation status at the promoter region of the gene encoding interleukin-6, 8 and 10 (IL-6, 8 and 10) was estimated. The levels of the corresponding gene products as well as DNA methyltransferases (DNMTs) quantity were assessed. Results: IL-1β decreased genomic methylation of human intestinal epithelial cells and induced demethylation at cg-specific sites at the promoter of pro-inflammatory genes IL6 and IL8; conversely it did not change the methylation of the IL10 promoter. IL-1β also increased the release of IL-6 and IL-8 but did not change the IL-10 expression. Finally, cell exposure to IL-1β decreased the DNMT3b expression, increased DNMT3a and was not able to change DNMT1 expression. Conclusions: Our results suggest a potential role of IL-1β as modulator of DNA methylation in activated differentiated Caco-2 cell line. | |
| Settore Scientifico Disciplinare: | Settore BIO/18 - Genetica Settore BIO/10 - Biochimica Settore BIO/13 - Biologia Applicata | |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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