The state of melatonin confined in dry cholesterol/lecithin mixed reversed micelles dispersed in CCl4 was investigated using H-1-NMR and FT-IR spectroscopies as a function of the melatonin to lecithin molar ratio (R-MLT) and of the cholesterol to lecithin molar ratio (R-CHL). An analysis of experimental results leads to the hypothesis that, independent of R-MLT and as a consequence of anisotropic melatonin/lecithin, melatonin/cholesterol and cholesterol/lecithin interactions, melatonin is totally solubilized in reversed micelles. Melatonin is mainly located in and oriented in the nanodomain constituted by the hydrophilic groups of cholesterol and lecithin. A competition of melatonin and cholesterol for the hydrophilic binding sites of the reversed micelles was observed by changing the R-CHL. Some possible biological implications of the specific interactions governing the solubilization process, the preferential location and the peculiar properties of melatonin confined in cholesterol/lecithin mixed reversed micelles are discussed.
|Data di pubblicazione:||2005|
|Titolo:||Localization and Interactions of Melatonin in Dry Cholesterol/Lecithin Mixed Reversed Micelles used as Cell Membrane Models|
|Autori:||BONGIORNO D; CERAULO L; FERRUGIA M; FILIZZOLA F; RUGGIRELLO A; TURCO LIVERI V|
|Tipologia:||Articolo su rivista|
|Citazione:||BONGIORNO D, CERAULO L, FERRUGIA M, FILIZZOLA F, RUGGIRELLO A, & TURCO LIVERI V (2005). Localization and Interactions of Melatonin in Dry Cholesterol/Lecithin Mixed Reversed Micelles used as Cell Membrane Models. JOURNAL OF PINEAL RESEARCH, 38, 292-298.|
|Digital Object Identifier (DOI):||10.1111/J.1600-79X.2005.00211.X|
|Appare nelle tipologie:||01 - Articolo su rivista|