We have previously isolated exosome-like nanoparticles from Citrus-limon juice, able to inhibit in vitro and in vivo tumor cell growth. In order to deeply understand the mechanism underlying nanovesicle effects, we performed a proteomic profile of treated colorectal cancer cells. Among the proteins differentially expressed after nanovesicle treatment, we found a significant downregulation of the Acetyl-CoA Carboxylase 1 (ACACA) and we demonstrated that silencing ACACA in cancer cells leads to a reduction of cell growth. Our study proved that the anti-tumor effects of Citrus-limon nanovesicles is partly mediated by lipid metabolism inhibition, in particular via ACACA downregulation.

Raimondo, S., Saieva, L., Cristaldi, M., Monteleone, F., Fontana, S., & Alessandro, R. (2018). Label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-CoA carboxylase alpha as antitumor target of Citrus limon-derived nanovesicles. JOURNAL OF PROTEOMICS, 173, 1-11 [10.1016/j.jprot.2017.11.017].

Label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-CoA carboxylase alpha as antitumor target of Citrus limon-derived nanovesicles

Raimondo, S.;Saieva, L.;Cristaldi, M.;Monteleone, F.;Fontana, S.;Alessandro, R.
2018

Abstract

We have previously isolated exosome-like nanoparticles from Citrus-limon juice, able to inhibit in vitro and in vivo tumor cell growth. In order to deeply understand the mechanism underlying nanovesicle effects, we performed a proteomic profile of treated colorectal cancer cells. Among the proteins differentially expressed after nanovesicle treatment, we found a significant downregulation of the Acetyl-CoA Carboxylase 1 (ACACA) and we demonstrated that silencing ACACA in cancer cells leads to a reduction of cell growth. Our study proved that the anti-tumor effects of Citrus-limon nanovesicles is partly mediated by lipid metabolism inhibition, in particular via ACACA downregulation.
Settore BIO/13 - Biologia Applicata
Raimondo, S., Saieva, L., Cristaldi, M., Monteleone, F., Fontana, S., & Alessandro, R. (2018). Label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-CoA carboxylase alpha as antitumor target of Citrus limon-derived nanovesicles. JOURNAL OF PROTEOMICS, 173, 1-11 [10.1016/j.jprot.2017.11.017].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/248970
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