The selection and validation of bioactive compounds require multiple approaches, including in-depth analyses of their biological activity in a whole-animal context. We exploited the sea urchin embryo in a rapid, medium-scale range screening to test the effects of the small synthetic kinase inhibitor kenpaullone. We show that sea urchin embryos specifically respond to this molecule depending on both dose and timing of administration. Phenotypic effects of kenpaullone are not immediately visible, since this molecule affects neither the fertilization nor the spatial arrangement of blastomeres at early developmental stages. Nevertheless, kenpaullone exposure from the beginning of embryogenesis profoundly perturbs specification, detachment from the epithelium, and migration of the primary mesenchyme cells, thus affecting the whole embryonic epithelial mesenchymal transition process. Our results reaffirm the sea urchin embryo as an excellent and sensitive in vivo system, which provides straightforward and rapid response to external stimuli.

Anello, L., Cavalieri, V., Di Bernardo, M. (2018). Developmental effects of the protein kinase inhibitor kenpaullone on the sea urchin embryo. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. C. TOXICOLOGY & PHARMACOLOGY, 204, 36-44 [10.1016/j.cbpc.2017.11.001].

Developmental effects of the protein kinase inhibitor kenpaullone on the sea urchin embryo

Cavalieri, Vincenzo;
2018-01-01

Abstract

The selection and validation of bioactive compounds require multiple approaches, including in-depth analyses of their biological activity in a whole-animal context. We exploited the sea urchin embryo in a rapid, medium-scale range screening to test the effects of the small synthetic kinase inhibitor kenpaullone. We show that sea urchin embryos specifically respond to this molecule depending on both dose and timing of administration. Phenotypic effects of kenpaullone are not immediately visible, since this molecule affects neither the fertilization nor the spatial arrangement of blastomeres at early developmental stages. Nevertheless, kenpaullone exposure from the beginning of embryogenesis profoundly perturbs specification, detachment from the epithelium, and migration of the primary mesenchyme cells, thus affecting the whole embryonic epithelial mesenchymal transition process. Our results reaffirm the sea urchin embryo as an excellent and sensitive in vivo system, which provides straightforward and rapid response to external stimuli.
2018
Anello, L., Cavalieri, V., Di Bernardo, M. (2018). Developmental effects of the protein kinase inhibitor kenpaullone on the sea urchin embryo. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. C. TOXICOLOGY & PHARMACOLOGY, 204, 36-44 [10.1016/j.cbpc.2017.11.001].
File in questo prodotto:
File Dimensione Formato  
Anello 2017 (Kp).pdf

Solo gestori archvio

Descrizione: articolo principale
Tipologia: Versione Editoriale
Dimensione 993.91 kB
Formato Adobe PDF
993.91 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/246518
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 2
social impact