gd T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties of tumor-infiltrating gd T cells and those in the corresponding normal tissue remain largely unknown. Here we have studied features of gd T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlated their levels with clinicopathologic hallmarks. Flow cytometry and transcriptome analyses showed that the tumor comprised a highly variable rate of TILs (5-90%) and 4%gd T cells on average, with the majority expressing Vd1. Most Vd1 and Vd2 T cells showed a predominant effector memory phenotype and had reduced production of IFN-g which was likely due to yet unidentified inhibitory molecules present in cancer stem cell secretome. Transcriptome analyses revealed that patients containing abundant gd T cells had significantly longer 5-year disease free survival rate, suggesting their efficacy in controlling tumor at very early stage.

Meraviglia, S., LO PRESTI, E., Tosolini, M., LA MENDOLA, C., Orlando, V., Todaro, M., et al. (2017). Distinctive features of tumor-infiltrating gd T lymphocytes in human colorectal cancer. ONCOIMMUNOLOGY, 6(10), 1347742-1-1347742-12 [10.1080/2162402X.2017.1347742].

Distinctive features of tumor-infiltrating gd T lymphocytes in human colorectal cancer

Meraviglia, S.
;
Lo Presti, E.;La Mendola, C.;Orlando, V.;Todaro, M.;Catalano, V.;Stassi, G.;Cicero, G.;Vieni, S.;Dieli, F.
2017

Abstract

gd T cells usually infiltrate many different types of cancer, but it is unclear whether they inhibit or promote tumor progression. Moreover, properties of tumor-infiltrating gd T cells and those in the corresponding normal tissue remain largely unknown. Here we have studied features of gd T cells in colorectal cancer, normal colon tissue and peripheral blood, and correlated their levels with clinicopathologic hallmarks. Flow cytometry and transcriptome analyses showed that the tumor comprised a highly variable rate of TILs (5-90%) and 4%gd T cells on average, with the majority expressing Vd1. Most Vd1 and Vd2 T cells showed a predominant effector memory phenotype and had reduced production of IFN-g which was likely due to yet unidentified inhibitory molecules present in cancer stem cell secretome. Transcriptome analyses revealed that patients containing abundant gd T cells had significantly longer 5-year disease free survival rate, suggesting their efficacy in controlling tumor at very early stage.
Settore MED/04 - Patologia Generale
https://www.tandfonline.com/doi/full/10.1080/2162402X.2017.1347742
Meraviglia, S., LO PRESTI, E., Tosolini, M., LA MENDOLA, C., Orlando, V., Todaro, M., et al. (2017). Distinctive features of tumor-infiltrating gd T lymphocytes in human colorectal cancer. ONCOIMMUNOLOGY, 6(10), 1347742-1-1347742-12 [10.1080/2162402X.2017.1347742].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10447/245773
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