BACKGROUND: Rhinovirus-induced acute asthma is the most frequent trigger for asthma exacerbations. OBJECTIVE: We assessed which inflammatory mediators were released from bronchial epithelial cells (BECs) after infection with rhinovirus and then determined whether they were also present in subjects with acute virus-induced asthma, with the aim to identify a biomarker or biomarkers for acute virus-induced asthma. METHODS: BECs were obtained from bronchial brushings of steroid-naive asthmatic subjects and healthy nonatopic control subjects. Cells were infected with rhinovirus 16. Inflammatory mediators were measured by means of flow cytometry with a cytometric bead array. Subjects with acute asthma and virus infection were recruited; they were characterized clinically by using lung function tests and had blood taken to measure the inflammatory mediators identified as important by the BEC experiments. RESULTS: IFN-gamma-induced protein 10 (IP-10) and RANTES were released in the greatest quantities, followed by IL-6, IL-8, and TNF-alpha. Dexamethasone treatment of BECs only partially suppressed IP-10 and TNF-alpha but was more effective at suppressing RANTES, IL-6, and IL-8. In acute clinical asthma serum IP-10 levels were increased to a greater extent in those with acute virus-induced asthma (median of 604 pg/mL compared with 167 pg/mL in those with non-virus-induced acute asthma, P < .01). Increased serum IP-10 levels were predictive of virus-induced asthma (odds ratio, 44.3 [95% CI, 3.9-100.3]). Increased serum IP-10 levels were strongly associated with more severe airflow obstruction (r = -0.8; P < .01). CONCLUSIONS: IP-10 release is specific to acute virus-induced asthma. CLINICAL IMPLICATIONS: Measurement of serum IP-10 could be used to predict a viral trigger to acute asthma.
|Data di pubblicazione:||2007|
|Titolo:||IFN-gamma-induced protein 10 is a novel biomarker of rhinovirus-induced asthma exacerbations|
|Autori:||WARK, PA; BUCCHIERI, F; JOHNSTON, SL; GIBSON, PG; HAMILTON, L; MIMICA, J; ZUMMO, G; HOLGATE, ST; ATTIA, J; THAKKINSTIAN, A; DAVIES, DE|
|Tipologia:||Articolo su rivista|
|Citazione:||WARK, P.A., BUCCHIERI, F., JOHNSTON, S.L., GIBSON, P.G., HAMILTON, L., MIMICA, J., et al. (2007). IFN-gamma-induced protein 10 is a novel biomarker of rhinovirus-induced asthma exacerbations. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 120(3), 586-593.|
|Tipo:||Articolo in rivista|
|Appare nelle tipologie:||01 - Articolo su rivista|