Profiling microbial communities in hyperalkaline waters of the Kizildag ophiolite complex (Turkey).

It has recently been hypothesized that life on Earth could have been originated in hyperalkaline waters related to serpentinization of ophiolitic rocks, despite their extreme conditions (high pH and very low levels of nutrients). Five hyperalkaline springs of the Kizildag ophiolite complex (Turkey) were characterized. The dominant gases are either H2, CH4 or N2 . Bacterial diversity, analysed by RISA (Ribosomal Intergenic Spacer Analysis) revealed different profles for each spring. Sequencing of excised DNA bands allowed to identify the presence of Bacillus, Ralstonia, Pseudoalteromonas., Ureibacillus, Alicycliphilus, Anaerococcus. 16S ribosomal DNA sequencing by Illumina is in progress. Three samples were also positive for the presence of pmoA (encoding the key enzyme of methane oxidation) confrming the presence of methanotrophs in accordance to gas analyses that showed clues of microbial methane oxidation in the isotopic ratio. The presence of methanotrophs in these hyperalkaline springs highlights the extraordinary capability of the methanotrophs to adapt to extreme conditions.