Study on Conversion of Encapsulated Vitro-Derived Propagules of C35 Citrange [C. sinensis (L.) Osb. × P. trifoliata (L.) Raf.]

Fight against Citrus tristeza virus (CTV) implies the use of CTV-resistant rootstocks, such as citranges [C. Sinensis (L.) Osb. × P. Trifoliata (L.) Raf.]. The 'C35' represents a promising tolerant to CTV genotype. The employment of innovative in vitro techniques, such as encapsulation, could open new perspectives for this genotype propagation, particularly in the nursery activity. Preliminary experiments on calcium-alginate encapsulation of microcuttings (unipolar propagules) have already been performed to evaluate the use of tissue culture for the propagation and the preservation of different citrange rootstocks: Carrizo and Troyer. In the present study, the influence of different types and concentration of auxins on the 'C35' encapsulated microcutting in vitro response was evaluated.