Polymer coated gold nanospheres are proposed as a tumor selective carrier for the anticancer drug doxorubicin. Thiolated polyethyleneglycol (PEG-SH) and an inulin-amino derivative based copolymer (INU-EDA) were used as stabilizing and coating materials for 40 nm gold nanospheres. The resulting polymer coated gold nanospheres (Au@PEG-INU) showed excellent physicochemical stability and potential stealth like behavior. The system was loaded with doxorubicin (Au@PEG-INU/Doxo) and its cytotoxicity profile was evaluated on human cervical cancer cells (HeLa) and lung cancer cells (A549), as compared to Au@PEG-INU and doxorubicin alone. Cytotoxicity assays showed that the system is able to drastically reduce cell viability upon incubation for 3 days. This result was supported by the ability of Au@PEG-INU/Doxo to be internalized by cancer cells and to release doxorubicin, as assessed by fluorescence microscopy. Finally, a cancer/non cancer cell co-culture model was used to display the advantageous therapeutic effects of the proposed system with respect to doxorubicin alone, thereby demonstrating the ability of Au@PEG-INU/Doxo to preferentially accumulate in tumor cells due to their enhanced metabolism, and to selectively kill target cells.

Li Volsi, A., Jimenez De Aberasturi, D., Henriksen-Lacey, M., Giammona, G., Licciardi, M., Liz-Marzán, L. (2016). Inulin coated plasmonic gold nanoparticles as a tumor-selective tool for cancer therapy. JOURNAL OF MATERIALS CHEMISTRY. B, 4(6), 1150-1155 [10.1039/c5tb01810b].

Inulin coated plasmonic gold nanoparticles as a tumor-selective tool for cancer therapy

Li Volsi, Anna;GIAMMONA, Gaetano;LICCIARDI, Mariano
;
2016-02-14

Abstract

Polymer coated gold nanospheres are proposed as a tumor selective carrier for the anticancer drug doxorubicin. Thiolated polyethyleneglycol (PEG-SH) and an inulin-amino derivative based copolymer (INU-EDA) were used as stabilizing and coating materials for 40 nm gold nanospheres. The resulting polymer coated gold nanospheres (Au@PEG-INU) showed excellent physicochemical stability and potential stealth like behavior. The system was loaded with doxorubicin (Au@PEG-INU/Doxo) and its cytotoxicity profile was evaluated on human cervical cancer cells (HeLa) and lung cancer cells (A549), as compared to Au@PEG-INU and doxorubicin alone. Cytotoxicity assays showed that the system is able to drastically reduce cell viability upon incubation for 3 days. This result was supported by the ability of Au@PEG-INU/Doxo to be internalized by cancer cells and to release doxorubicin, as assessed by fluorescence microscopy. Finally, a cancer/non cancer cell co-culture model was used to display the advantageous therapeutic effects of the proposed system with respect to doxorubicin alone, thereby demonstrating the ability of Au@PEG-INU/Doxo to preferentially accumulate in tumor cells due to their enhanced metabolism, and to selectively kill target cells.
14-feb-2016
Li Volsi, A., Jimenez De Aberasturi, D., Henriksen-Lacey, M., Giammona, G., Licciardi, M., Liz-Marzán, L. (2016). Inulin coated plasmonic gold nanoparticles as a tumor-selective tool for cancer therapy. JOURNAL OF MATERIALS CHEMISTRY. B, 4(6), 1150-1155 [10.1039/c5tb01810b].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10447/230384
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